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Detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages, a feasibility study

Eijsink, J. J. H., Yang, N., Lendvai, A., Klip, H. G., Volders, H. H., Buikema, H. J., van Hemel, B. M., Voll, M., Bennink, H. J. T. C., Schuuring, E., Wisman, G. B. A. & van der Zee, A. G. J., Feb-2011, In : Gynecologic Oncology. 120, 2, p. 280-283 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

Objective. To explore the feasibility of DNA methylation analysis for the detection of cervical neoplasia in self-obtained cervico-vaginal lavages.

Methods. Lavages collected by a self-sampling device and paired cervical scrapings were obtained from 20 cervical cancer patients and 23 patients referred with an abnormal cervical smear (15 with high-grade cervical intraepithelial neoplasia (CIN2+) and 8 without CIN). All lavages and scrapings were analyzed by liquid based cytology (LBC), Hybrid Capture II (HC-II) for hr-HPV DNA detection and by DNA methylation analysis (JAM3, TERT, EPB41L3 and C13ORF18). Concordance between lavages and scrapings was measured by Cohen's Kappa (k).

Results. In lavages and scrapings from cervical cancer patients (n =20), methylation analysis was positive in 19 (95%) and 19 (95%), HC-II in 16 (80%) and 15 (75%) and LBC in 15 (75%) and 19 (95%), respectively. In lavages and scrapings from CIN2+ patients (n = 15), methylation analysis was positive in 10 (67%) and 12 (80%), HC-II in 15 (100%) and 15 (100%) and LBC in 11 (73%) and 12 (80%), respectively. Concordance between cervical scrapings and lavages (n = 43) was for LBC k = 0.522 (p

Conclusions. DNA methylation analysis in cervico-vaginal lavages obtained by a self-sampling device is feasible and its diagnostic performance appears to be at least comparable to the detection of cervical neoplasia by cytomorphology and hr-HPV. Our pilot study suggests that detection of cervical neoplasia by DNA methylation analysis in cervico-vaginal lavages warrants exploration of its use in large prospective studies. (C) 2010 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)280-283
Number of pages4
JournalGynecologic Oncology
Volume120
Issue number2
Publication statusPublished - Feb-2011

    Keywords

  • Cervical, Neoplasia, Self-sampling, Lavage, Cervical cancer, Methylation markers, HUMAN-PAPILLOMAVIRUS DNA, INTRAEPITHELIAL NEOPLASIA, CANCER, NETHERLANDS, CYTOLOGY, MARKERS, WOMEN

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