Publication

Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution

Ravelli, R. B. G., Kalicharan, R. D., Avramut, M. C., Sjollema, K. A., Pronk, J. W., Dijk, F., Koster, A. J., Visser, J. T. J., Faas, F. G. A. & Giepmans, B. N. G., 8-May-2013, In : Scientific Reports. 3, 6 p., 1804.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ravelli, R. B. G., Kalicharan, R. D., Avramut, M. C., Sjollema, K. A., Pronk, J. W., Dijk, F., ... Giepmans, B. N. G. (2013). Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution. Scientific Reports, 3, [1804]. https://doi.org/10.1038/srep01804

Author

Ravelli, Raimond B. G. ; Kalicharan, Ruby D. ; Avramut, M. Cristina ; Sjollema, Klaas A. ; Pronk, Joachim W. ; Dijk, Freark ; Koster, Abraham J. ; Visser, Jeroen T. J. ; Faas, Frank G. A. ; Giepmans, Ben N. G. / Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution. In: Scientific Reports. 2013 ; Vol. 3.

Harvard

Ravelli, RBG, Kalicharan, RD, Avramut, MC, Sjollema, KA, Pronk, JW, Dijk, F, Koster, AJ, Visser, JTJ, Faas, FGA & Giepmans, BNG 2013, 'Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution' Scientific Reports, vol. 3, 1804. https://doi.org/10.1038/srep01804

Standard

Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution. / Ravelli, Raimond B. G.; Kalicharan, Ruby D.; Avramut, M. Cristina; Sjollema, Klaas A.; Pronk, Joachim W.; Dijk, Freark; Koster, Abraham J.; Visser, Jeroen T. J.; Faas, Frank G. A.; Giepmans, Ben N. G.

In: Scientific Reports, Vol. 3, 1804, 08.05.2013.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ravelli RBG, Kalicharan RD, Avramut MC, Sjollema KA, Pronk JW, Dijk F et al. Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution. Scientific Reports. 2013 May 8;3. 1804. https://doi.org/10.1038/srep01804


BibTeX

@article{1a99865a4b6c4dc4a8301947b9b33751,
title = "Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution",
abstract = "Finding alternatives for insulin therapy and making advances in etiology of type 1 diabetes benefits from a full structural and functional insight into Islets of Langerhans. Electron microscopy (EM) can visualize Islet morphology at the highest possible resolution, however, conventional EM only provides biased snapshots and lacks context. We developed and employed large scale EM and compiled a resource of complete cross sections of rat Islets during immuno-destruction to provide unbiased structural insight of thousands of cells at macromolecular resolution. The resource includes six datasets, totalling 25.000 micrographs, annotated for cellular and ultrastructural changes during autoimmune diabetes. Granulocytes are attracted to the endocrine tissue, followed by extravasation of a pleiotrophy of leukocytes. Subcellullar changes in beta cells include endoplasmic reticulum stress, insulin degranulation and glycogen accumulation. Rare findings include erythrocyte extravasation and nuclear actin-like fibers. While we focus on a rat model of autoimmune diabetes, our approach is general applicable.",
keywords = "PRONE BB RATS, ELECTRON-MICROSCOPY, PANCREATIC-ISLETS, BETA-CELL, LJUNGAN VIRUS, INSULITIS, INFLAMMATION, LOCALIZATION, LANGERHANS, INFECTION",
author = "Ravelli, {Raimond B. G.} and Kalicharan, {Ruby D.} and Avramut, {M. Cristina} and Sjollema, {Klaas A.} and Pronk, {Joachim W.} and Freark Dijk and Koster, {Abraham J.} and Visser, {Jeroen T. J.} and Faas, {Frank G. A.} and Giepmans, {Ben N. G.}",
year = "2013",
month = "5",
day = "8",
doi = "10.1038/srep01804",
language = "English",
volume = "3",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution

AU - Ravelli, Raimond B. G.

AU - Kalicharan, Ruby D.

AU - Avramut, M. Cristina

AU - Sjollema, Klaas A.

AU - Pronk, Joachim W.

AU - Dijk, Freark

AU - Koster, Abraham J.

AU - Visser, Jeroen T. J.

AU - Faas, Frank G. A.

AU - Giepmans, Ben N. G.

PY - 2013/5/8

Y1 - 2013/5/8

N2 - Finding alternatives for insulin therapy and making advances in etiology of type 1 diabetes benefits from a full structural and functional insight into Islets of Langerhans. Electron microscopy (EM) can visualize Islet morphology at the highest possible resolution, however, conventional EM only provides biased snapshots and lacks context. We developed and employed large scale EM and compiled a resource of complete cross sections of rat Islets during immuno-destruction to provide unbiased structural insight of thousands of cells at macromolecular resolution. The resource includes six datasets, totalling 25.000 micrographs, annotated for cellular and ultrastructural changes during autoimmune diabetes. Granulocytes are attracted to the endocrine tissue, followed by extravasation of a pleiotrophy of leukocytes. Subcellullar changes in beta cells include endoplasmic reticulum stress, insulin degranulation and glycogen accumulation. Rare findings include erythrocyte extravasation and nuclear actin-like fibers. While we focus on a rat model of autoimmune diabetes, our approach is general applicable.

AB - Finding alternatives for insulin therapy and making advances in etiology of type 1 diabetes benefits from a full structural and functional insight into Islets of Langerhans. Electron microscopy (EM) can visualize Islet morphology at the highest possible resolution, however, conventional EM only provides biased snapshots and lacks context. We developed and employed large scale EM and compiled a resource of complete cross sections of rat Islets during immuno-destruction to provide unbiased structural insight of thousands of cells at macromolecular resolution. The resource includes six datasets, totalling 25.000 micrographs, annotated for cellular and ultrastructural changes during autoimmune diabetes. Granulocytes are attracted to the endocrine tissue, followed by extravasation of a pleiotrophy of leukocytes. Subcellullar changes in beta cells include endoplasmic reticulum stress, insulin degranulation and glycogen accumulation. Rare findings include erythrocyte extravasation and nuclear actin-like fibers. While we focus on a rat model of autoimmune diabetes, our approach is general applicable.

KW - PRONE BB RATS

KW - ELECTRON-MICROSCOPY

KW - PANCREATIC-ISLETS

KW - BETA-CELL

KW - LJUNGAN VIRUS

KW - INSULITIS

KW - INFLAMMATION

KW - LOCALIZATION

KW - LANGERHANS

KW - INFECTION

U2 - 10.1038/srep01804

DO - 10.1038/srep01804

M3 - Article

VL - 3

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 1804

ER -

ID: 5859961