Publication

Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum

Guzman-Chavez, F., Salo, O., Samol, M., Ries, M., Kuipers, J., Bovenberg, R. A. L., Vreeken, R. J. & Driessen, A. J. M., Oct-2018, In : MicrobiologyOpen. 7, 5, 15 p., e00598.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Guzman-Chavez, F., Salo, O., Samol, M., Ries, M., Kuipers, J., Bovenberg, R. A. L., ... Driessen, A. J. M. (2018). Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum. MicrobiologyOpen, 7(5), [e00598]. https://doi.org/10.1002/mbo3.598

Author

Guzman-Chavez, Fernando ; Salo, Oleksandr ; Samol, Marta ; Ries, Marco ; Kuipers, Jeroen ; Bovenberg, Roel A L ; Vreeken, Rob J ; Driessen, Arnold J M. / Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum. In: MicrobiologyOpen. 2018 ; Vol. 7, No. 5.

Harvard

Guzman-Chavez, F, Salo, O, Samol, M, Ries, M, Kuipers, J, Bovenberg, RAL, Vreeken, RJ & Driessen, AJM 2018, 'Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum', MicrobiologyOpen, vol. 7, no. 5, e00598. https://doi.org/10.1002/mbo3.598

Standard

Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum. / Guzman-Chavez, Fernando; Salo, Oleksandr; Samol, Marta; Ries, Marco; Kuipers, Jeroen; Bovenberg, Roel A L; Vreeken, Rob J; Driessen, Arnold J M.

In: MicrobiologyOpen, Vol. 7, No. 5, e00598, 10.2018.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Guzman-Chavez F, Salo O, Samol M, Ries M, Kuipers J, Bovenberg RAL et al. Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum. MicrobiologyOpen. 2018 Oct;7(5). e00598. https://doi.org/10.1002/mbo3.598


BibTeX

@article{c9dc80b8e7af42d1a2eafc77a25c2b5c,
title = "Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum",
abstract = "The Pc21 g14570 gene of Penicillium chrysogenum encodes an ortholog of a class 2 histone deacetylase termed HdaA which may play a role in epigenetic regulation of secondary metabolism. Deletion of the hdaA gene induces a significant pleiotropic effect on the expression of a set of polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS)-encoding genes. The deletion mutant exhibits a decreased conidial pigmentation that is related to a reduced expression of the PKS gene Pc21 g16000 (pks17) responsible for the production of the pigment precursor naphtha-γ-pyrone. Moreover, the hdaA deletion caused decreased levels of the yellow pigment chrysogine that is associated with the downregulation of the NRPS-encoding gene Pc21 g12630 and associated biosynthetic gene cluster. In contrast, transcriptional activation of the sorbicillinoids biosynthetic gene cluster occurred concomitantly with the overproduction of associated compounds . A new compound was detected in the deletion strain that was observed only under conditions of sorbicillinoids production, suggesting crosstalk between biosynthetic gene clusters. Our present results show that an epigenomic approach can be successfully applied for the activation of secondary metabolism in industrial strains of P. chrysogenum.",
keywords = "chrysogine, crosstalk, histone deacetylase, naphtha-gamma-pyrone, Penicillium chrysogenum, sorbicillinoids",
author = "Fernando Guzman-Chavez and Oleksandr Salo and Marta Samol and Marco Ries and Jeroen Kuipers and Bovenberg, {Roel A L} and Vreeken, {Rob J} and Driessen, {Arnold J M}",
note = "{\circledC} 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2018",
month = "10",
doi = "10.1002/mbo3.598",
language = "English",
volume = "7",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "Wiley",
number = "5",

}

RIS

TY - JOUR

T1 - Deregulation of secondary metabolism in a histone deacetylase mutant of Penicillium chrysogenum

AU - Guzman-Chavez, Fernando

AU - Salo, Oleksandr

AU - Samol, Marta

AU - Ries, Marco

AU - Kuipers, Jeroen

AU - Bovenberg, Roel A L

AU - Vreeken, Rob J

AU - Driessen, Arnold J M

N1 - © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

PY - 2018/10

Y1 - 2018/10

N2 - The Pc21 g14570 gene of Penicillium chrysogenum encodes an ortholog of a class 2 histone deacetylase termed HdaA which may play a role in epigenetic regulation of secondary metabolism. Deletion of the hdaA gene induces a significant pleiotropic effect on the expression of a set of polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS)-encoding genes. The deletion mutant exhibits a decreased conidial pigmentation that is related to a reduced expression of the PKS gene Pc21 g16000 (pks17) responsible for the production of the pigment precursor naphtha-γ-pyrone. Moreover, the hdaA deletion caused decreased levels of the yellow pigment chrysogine that is associated with the downregulation of the NRPS-encoding gene Pc21 g12630 and associated biosynthetic gene cluster. In contrast, transcriptional activation of the sorbicillinoids biosynthetic gene cluster occurred concomitantly with the overproduction of associated compounds . A new compound was detected in the deletion strain that was observed only under conditions of sorbicillinoids production, suggesting crosstalk between biosynthetic gene clusters. Our present results show that an epigenomic approach can be successfully applied for the activation of secondary metabolism in industrial strains of P. chrysogenum.

AB - The Pc21 g14570 gene of Penicillium chrysogenum encodes an ortholog of a class 2 histone deacetylase termed HdaA which may play a role in epigenetic regulation of secondary metabolism. Deletion of the hdaA gene induces a significant pleiotropic effect on the expression of a set of polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS)-encoding genes. The deletion mutant exhibits a decreased conidial pigmentation that is related to a reduced expression of the PKS gene Pc21 g16000 (pks17) responsible for the production of the pigment precursor naphtha-γ-pyrone. Moreover, the hdaA deletion caused decreased levels of the yellow pigment chrysogine that is associated with the downregulation of the NRPS-encoding gene Pc21 g12630 and associated biosynthetic gene cluster. In contrast, transcriptional activation of the sorbicillinoids biosynthetic gene cluster occurred concomitantly with the overproduction of associated compounds . A new compound was detected in the deletion strain that was observed only under conditions of sorbicillinoids production, suggesting crosstalk between biosynthetic gene clusters. Our present results show that an epigenomic approach can be successfully applied for the activation of secondary metabolism in industrial strains of P. chrysogenum.

KW - chrysogine

KW - crosstalk

KW - histone deacetylase

KW - naphtha-gamma-pyrone

KW - Penicillium chrysogenum

KW - sorbicillinoids

U2 - 10.1002/mbo3.598

DO - 10.1002/mbo3.598

M3 - Article

C2 - 29575742

VL - 7

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 5

M1 - e00598

ER -

ID: 56240130