Publication

Deletion of cdvB paralogous genes of Sulfolobus acidocaldarius impairs cell division

Yang, N. & Driessen, A. J. M., 2014, In : Extremophiles. 18, 2, p. 331-339 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Deletion of cdvB paralogous genes of Sulfolobus acidocaldarius impairs cell division

    Final publisher's version, 5.31 MB, PDF document

    Request copy

DOI

The majority of Crenarchaeota utilize the cell division system (Cdv) to divide. This system consists of three highly conserved genes, cdvA, cdvB and cdvC that are organized in an operon. CdvC is homologous to the AAA-type ATPase Vps4, involved in multivesicular body biogenesis in eukaryotes. CdvA is a unique archaeal protein that interacts with the membrane, while CdvB is homologous to the eukaryal Vps24 and forms helical filaments. Most Crenarcheota contain additional CdvB paralogs. In Sulfolobus acidocaldarius these are termed CdvB1–3. We have used a gene inactivation approach to determine the impact of these additional cdvB genes on cell division. Independent deletion mutants of these genes were analyzed for growth and protein localization. One of the deletion strains (DcdvB3) showed a severe growth defect on plates and delayed growth on liquid medium. It showed the formation of enlarged cells and a defect in DNA segregation. Since these defects are accompanied with an aberrant localization of CdvA and CdvB, we conclude that CdvB3 fulfills an important accessory role in cell division.
Original languageEnglish
Pages (from-to)331-339
Number of pages9
JournalExtremophiles
Volume18
Issue number2
Publication statusPublished - 2014

    Keywords

  • Archaea, Sulfolobales, Cell division, TURRETED ICOSAHEDRAL VIRUS, ESCRT MACHINERY, STRUCTURAL BASIS, FTSZ RING, ARCHAEA, PROTEINS, VPS4, SCISSION, ATPASE, SOLFATARICUS

ID: 2339767