Defining the Region of Bacillus subtilis SpoIIIJ That Is Essential for Its Sporulation-Specific FunctionGeng, Y., Keyzer, J. D., Scheffers, D-J. & Driessen, A. J. M., 2014, In : Journal of Bacteriology. 196, 7, p. 1318-1324 7 p.
Research output: Contribution to journal › Article › Academic › peer-review
Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporulation, while YqjG stimulates competence development. To obtain more insight into the regions important for the sporulation specificity of SpoIIIJ, a series of SpoIIIJ/YqjG chimeras was constructed. These chimeras were tested for functionality during vegetative growth and for their ability to complement the sporulation defect of a spoIIIJ deletion strain. The data suggest an important role for the domain comprising transmembrane segment 2 (TMS2) and its flanking loops in sporulation specificity, with lesser contributions to specificity by TMS1 and TMS3.
|Number of pages||7|
|Journal||Journal of Bacteriology|
|Publication status||Published - 2014|
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