Defining the Region of Bacillus subtilis SpoIIIJ That Is Essential for Its Sporulation-Specific Function

Geng, Y., Keyzer, J. D., Scheffers, D-J. & Driessen, A. J. M., 2014, In : Journal of Bacteriology. 196, 7, p. 1318-1324 7 p.

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Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporulation, while YqjG stimulates competence development. To obtain more insight into the regions important for the sporulation specificity of SpoIIIJ, a series of SpoIIIJ/YqjG chimeras was constructed. These chimeras were tested for functionality during vegetative growth and for their ability to complement the sporulation defect of a spoIIIJ deletion strain. The data suggest an important role for the domain comprising transmembrane segment 2 (TMS2) and its flanking loops in sporulation specificity, with lesser contributions to specificity by TMS1 and TMS3.
Original languageEnglish
Pages (from-to)1318-1324
Number of pages7
JournalJournal of Bacteriology
Issue number7
Publication statusPublished - 2014

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