Publication

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

Martens, D. E., Nollen, E. A., Hardeveld, M., Velden-de Groot, C. A., Gooijer, C. D., Beuvery, E. C. & Tramper, J., Jan-1997, In : Cytotechnology. 23, 1, p. 61-75 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Martens, D. E., Nollen, E. A., Hardeveld, M., Velden-de Groot, C. A., Gooijer, C. D., Beuvery, E. C., & Tramper, J. (1997). Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers. Cytotechnology, 23(1), 61-75. https://doi.org/10.1023/B:CYTO.0000010399.23373.65

Author

Martens, D E ; Nollen, E A ; Hardeveld, M ; Velden-de Groot, C A ; Gooijer, C D ; Beuvery, E C ; Tramper, J. / Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers. In: Cytotechnology. 1997 ; Vol. 23, No. 1. pp. 61-75.

Harvard

Martens, DE, Nollen, EA, Hardeveld, M, Velden-de Groot, CA, Gooijer, CD, Beuvery, EC & Tramper, J 1997, 'Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers', Cytotechnology, vol. 23, no. 1, pp. 61-75. https://doi.org/10.1023/B:CYTO.0000010399.23373.65

Standard

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers. / Martens, D E; Nollen, E A; Hardeveld, M; Velden-de Groot, C A; Gooijer, C D; Beuvery, E C; Tramper, J.

In: Cytotechnology, Vol. 23, No. 1, 01.1997, p. 61-75.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Martens DE, Nollen EA, Hardeveld M, Velden-de Groot CA, Gooijer CD, Beuvery EC et al. Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers. Cytotechnology. 1997 Jan;23(1):61-75. https://doi.org/10.1023/B:CYTO.0000010399.23373.65


BibTeX

@article{aa9e056b2b6f45c18145e962cbc19b1a,
title = "Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers",
abstract = "The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.",
keywords = "Journal Article",
author = "Martens, {D E} and Nollen, {E A} and M Hardeveld and {Velden-de Groot}, {C A} and Gooijer, {C D} and Beuvery, {E C} and J Tramper",
year = "1997",
month = "1",
doi = "10.1023/B:CYTO.0000010399.23373.65",
language = "English",
volume = "23",
pages = "61--75",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "SPRINGER",
number = "1",

}

RIS

TY - JOUR

T1 - Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

AU - Martens, D E

AU - Nollen, E A

AU - Hardeveld, M

AU - Velden-de Groot, C A

AU - Gooijer, C D

AU - Beuvery, E C

AU - Tramper, J

PY - 1997/1

Y1 - 1997/1

N2 - The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.

AB - The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.

KW - Journal Article

U2 - 10.1023/B:CYTO.0000010399.23373.65

DO - 10.1023/B:CYTO.0000010399.23373.65

M3 - Article

VL - 23

SP - 61

EP - 75

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 1

ER -

ID: 40681641