Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriersMartens, D. E., Nollen, E. A., Hardeveld, M., Velden-de Groot, C. A., Gooijer, C. D., Beuvery, E. C. & Tramper, J., Jan-1997, In : Cytotechnology. 23, 1, p. 61-75 15 p.
Research output: Contribution to journal › Article › Academic › peer-review
The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.
|Number of pages||15|
|Publication status||Published - Jan-1997|
- Journal Article