Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

Martens, D. E., Nollen, E. A., Hardeveld, M., Velden-de Groot, C. A., Gooijer, C. D., Beuvery, E. C. & Tramper, J., Jan-1997, In : Cytotechnology. 23, 1, p. 61-75 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • D E Martens
  • E A Nollen
  • M Hardeveld
  • C A Velden-de Groot
  • C D Gooijer
  • E C Beuvery
  • J Tramper

The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.

Original languageEnglish
Pages (from-to)61-75
Number of pages15
Issue number1
Publication statusPublished - Jan-1997


  • Journal Article

ID: 40681641