De novo rational design of a freestanding, supercharged polypeptide, proton-conducting membraneMa, C., Dong, J., Viviani, M., Tulini, I., Pontillo, N., Maity, S., Zhou, Y., Roos, W. H., Liu, K., Herrmann, A. & Portale, G., Jul-2020, In : Science Advances. 6, 29, 9 p., 0810.
Research output: Contribution to journal › Article › Academic › peer-review
Proton translocation enables important processes in nature and man-made technologies. However, controlling proton conduction and fabrication of devices exploiting biomaterials remains a challenge. Even more difficult is the design of protein-based bulk materials without any functional starting scaffold for further optimization. Here, we show the rational design of proton-conducting, protein materials exceeding reported proteinaceous systems. The carboxylic acid-rich structures were evolved step by step by exploring various sequences from intrinsically disordered coils over supercharged nanobarrels to hierarchically spider β sheet containing protein-supercharged polypeptide chimeras. The latter material is characterized by interconnected β sheet nanodomains decorated on their surface by carboxylic acid groups, forming self-supportive membranes and allowing for proton conduction in the hydrated state. The membranes showed an extraordinary proton conductivity of 18.5 ± 5 mS/cm at RH = 90%, one magnitude higher than other protein devices. This design paradigm offers great potential for bioprotonic device fabrication interfacing artificial and biological systems.
|Number of pages||9|
|Publication status||Published - Jul-2020|
- SPIDER SILK, GROTTHUSS, EVOLUTION, TRANSPORT, PROTEINS, WATER