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Damaged peroxisomes are subject to rapid autophagic degradation in the yeast Hansenula polymorpha

van Zutphen, T., Veenhuis, M. & van der Klei, I. J., Aug-2011, In : Autophagy. 7, 8, p. 863-872 10 p.

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DOI

Evidence is accumulating that damaged components of eukaryotic cells are removed by autophagic degradation (e. g., mitophagy). Here we show that peroxisomes that are damaged by the abrupt removal of the membrane protein Pex3 are massively and rapidly degraded even when the cells are placed at peroxisome-inducing conditions and hence need the organelles for growth. Pex3 degradation was induced by a temperature shift using Hansenula polymorpha pex3 Delta cells producing a Pex3 fusion protein containing an N-terminal temperature sensitive degron sequence. The massive peroxisome degradation process, associated with Pex3 degradation, showed properties of both micro-and macropexophagy and was dependent on Atg1 and Ypt7. This mode of peroxisome degradation is of physiological significance as it was also observed at conditions that excessive ROS is formed from peroxisome metabolism, i.e., when methanol-grown wild-type cells are exposed to methanol excess conditions.

Original languageEnglish
Pages (from-to)863-872
Number of pages10
JournalAutophagy
Volume7
Issue number8
Publication statusPublished - Aug-2011

    Keywords

  • Pex3, degron, pexophagy, autophagy, Hansenula polymorpha, SACCHAROMYCES-CEREVISIAE, SELECTIVE DEGRADATION, PICHIA-PASTORIS, GENE ENCODES, PROTEIN, MACROPEXOPHAGY, PEXOPHAGY, CELLS, PEX3P, MITOCHONDRIA

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