Damaged peroxisomes are subject to rapid autophagic degradation in the yeast Hansenula polymorphavan Zutphen, T., Veenhuis, M. & van der Klei, I. J., Aug-2011, In : Autophagy. 7, 8, p. 863-872 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
Evidence is accumulating that damaged components of eukaryotic cells are removed by autophagic degradation (e. g., mitophagy). Here we show that peroxisomes that are damaged by the abrupt removal of the membrane protein Pex3 are massively and rapidly degraded even when the cells are placed at peroxisome-inducing conditions and hence need the organelles for growth. Pex3 degradation was induced by a temperature shift using Hansenula polymorpha pex3 Delta cells producing a Pex3 fusion protein containing an N-terminal temperature sensitive degron sequence. The massive peroxisome degradation process, associated with Pex3 degradation, showed properties of both micro-and macropexophagy and was dependent on Atg1 and Ypt7. This mode of peroxisome degradation is of physiological significance as it was also observed at conditions that excessive ROS is formed from peroxisome metabolism, i.e., when methanol-grown wild-type cells are exposed to methanol excess conditions.
|Number of pages||10|
|Publication status||Published - Aug-2011|
- Pex3, degron, pexophagy, autophagy, Hansenula polymorpha, SACCHAROMYCES-CEREVISIAE, SELECTIVE DEGRADATION, PICHIA-PASTORIS, GENE ENCODES, PROTEIN, MACROPEXOPHAGY, PEXOPHAGY, CELLS, PEX3P, MITOCHONDRIA