Publication

CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus

van Weering, H. R. J., Boddeke, H. W. G. M., Vinet, J., Brouwer, N., de Haas, A. H., van Rooijen, N., Thomsen, A. R. & Biber, K. P. H., 2011, In : Hippocampus. 21, 2, p. 220-232 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van Weering, H. R. J., Boddeke, H. W. G. M., Vinet, J., Brouwer, N., de Haas, A. H., van Rooijen, N., Thomsen, A. R., & Biber, K. P. H. (2011). CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus. Hippocampus, 21(2), 220-232. https://doi.org/10.1002/hipo.20742

Author

van Weering, Hilmar R. J. ; Boddeke, Hendrikus W. G. M. ; Vinet, Jonathan ; Brouwer, Nieske ; de Haas, Alexander H. ; van Rooijen, Nico ; Thomsen, Allan R. ; Biber, Knut P. H. / CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus. In: Hippocampus. 2011 ; Vol. 21, No. 2. pp. 220-232.

Harvard

van Weering, HRJ, Boddeke, HWGM, Vinet, J, Brouwer, N, de Haas, AH, van Rooijen, N, Thomsen, AR & Biber, KPH 2011, 'CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus', Hippocampus, vol. 21, no. 2, pp. 220-232. https://doi.org/10.1002/hipo.20742

Standard

CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus. / van Weering, Hilmar R. J.; Boddeke, Hendrikus W. G. M.; Vinet, Jonathan; Brouwer, Nieske; de Haas, Alexander H.; van Rooijen, Nico; Thomsen, Allan R.; Biber, Knut P. H.

In: Hippocampus, Vol. 21, No. 2, 2011, p. 220-232.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van Weering HRJ, Boddeke HWGM, Vinet J, Brouwer N, de Haas AH, van Rooijen N et al. CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus. Hippocampus. 2011;21(2):220-232. https://doi.org/10.1002/hipo.20742


BibTeX

@article{b05dff1e513f43f2aabfb55e7b7b52ae,
title = "CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus",
abstract = "The chemokine CXCL10 and its receptor CXCR3 are implicated in various CNS pathologies since interference with CXCL10/CXCR3 signaling alters the onset and progression in various CNS disease models. However, the mechanism and cell-types involved in CXCL10/CXCR3 signaling under pathological conditions are far from understood. Here, we investigated the potential role for CXCL10/CXCR3 signaling in neuronal cell death and glia activation in response to N-methyl-D-aspartic acid (NMDA)-induced excitotoxicity in mouse organotypic hippocampal slice cultures (OHSCs). Our findings demonstrate that astrocytes express CXCL10 in response to excitotoxicity. Experiments in OHSCs derived from CXCL10-deficient (CXCL10(-/-)) and CXCR3-deficient (CXCR3(-/-)) revealed that in the absence of CXCL10 or CXCR3, neuronal cell death in the CA1 and CA3 regions was diminished after NMDA-treatment when compared to wild type OHSCs. In contrast, neuronal cell death in the DG region was enhanced in both CXCL10(-/-) and CXCR3(-/-) OHSCs in response to a high (50 mu M) NMDA-concentration. Moreover, we show that in the absence of microglia the differential changes in neuronal vulnerability between CXCR3(-/-) and wild type OHSCs are fully abrogated and therefore a prominent role for microglia in this process is suggested. Taken together, our results identify a region-specific role for CXCL10/CXCR3 signaling in neuron-glia and glia-glia interactions under pathological conditions. (C) 2010 Wiley-Liss, Inc.",
keywords = "chemokines, excitotoxicity, astrocyte, microglia, liposomic clodronate, CENTRAL-NERVOUS-SYSTEM, EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS, CHEMOKINE RECEPTOR CXCR3, MULTIPLE-SCLEROSIS, SLICE CULTURES, IFN-GAMMA, MICROGLIAL CELLS, INDUCIBLE PROTEIN-10, MESSENGER-RNA, GLUTAMATE NEUROTOXICITY",
author = "{van Weering}, {Hilmar R. J.} and Boddeke, {Hendrikus W. G. M.} and Jonathan Vinet and Nieske Brouwer and {de Haas}, {Alexander H.} and {van Rooijen}, Nico and Thomsen, {Allan R.} and Biber, {Knut P. H.}",
year = "2011",
doi = "10.1002/hipo.20742",
language = "English",
volume = "21",
pages = "220--232",
journal = "Hippocampus",
issn = "1050-9631",
publisher = "WILEY-LISS",
number = "2",

}

RIS

TY - JOUR

T1 - CXCL10/CXCR3 Signaling in Glia Cells Differentially Affects NMDA-Induced Cell Death in CA and DG Neurons of the Mouse Hippocampus

AU - van Weering, Hilmar R. J.

AU - Boddeke, Hendrikus W. G. M.

AU - Vinet, Jonathan

AU - Brouwer, Nieske

AU - de Haas, Alexander H.

AU - van Rooijen, Nico

AU - Thomsen, Allan R.

AU - Biber, Knut P. H.

PY - 2011

Y1 - 2011

N2 - The chemokine CXCL10 and its receptor CXCR3 are implicated in various CNS pathologies since interference with CXCL10/CXCR3 signaling alters the onset and progression in various CNS disease models. However, the mechanism and cell-types involved in CXCL10/CXCR3 signaling under pathological conditions are far from understood. Here, we investigated the potential role for CXCL10/CXCR3 signaling in neuronal cell death and glia activation in response to N-methyl-D-aspartic acid (NMDA)-induced excitotoxicity in mouse organotypic hippocampal slice cultures (OHSCs). Our findings demonstrate that astrocytes express CXCL10 in response to excitotoxicity. Experiments in OHSCs derived from CXCL10-deficient (CXCL10(-/-)) and CXCR3-deficient (CXCR3(-/-)) revealed that in the absence of CXCL10 or CXCR3, neuronal cell death in the CA1 and CA3 regions was diminished after NMDA-treatment when compared to wild type OHSCs. In contrast, neuronal cell death in the DG region was enhanced in both CXCL10(-/-) and CXCR3(-/-) OHSCs in response to a high (50 mu M) NMDA-concentration. Moreover, we show that in the absence of microglia the differential changes in neuronal vulnerability between CXCR3(-/-) and wild type OHSCs are fully abrogated and therefore a prominent role for microglia in this process is suggested. Taken together, our results identify a region-specific role for CXCL10/CXCR3 signaling in neuron-glia and glia-glia interactions under pathological conditions. (C) 2010 Wiley-Liss, Inc.

AB - The chemokine CXCL10 and its receptor CXCR3 are implicated in various CNS pathologies since interference with CXCL10/CXCR3 signaling alters the onset and progression in various CNS disease models. However, the mechanism and cell-types involved in CXCL10/CXCR3 signaling under pathological conditions are far from understood. Here, we investigated the potential role for CXCL10/CXCR3 signaling in neuronal cell death and glia activation in response to N-methyl-D-aspartic acid (NMDA)-induced excitotoxicity in mouse organotypic hippocampal slice cultures (OHSCs). Our findings demonstrate that astrocytes express CXCL10 in response to excitotoxicity. Experiments in OHSCs derived from CXCL10-deficient (CXCL10(-/-)) and CXCR3-deficient (CXCR3(-/-)) revealed that in the absence of CXCL10 or CXCR3, neuronal cell death in the CA1 and CA3 regions was diminished after NMDA-treatment when compared to wild type OHSCs. In contrast, neuronal cell death in the DG region was enhanced in both CXCL10(-/-) and CXCR3(-/-) OHSCs in response to a high (50 mu M) NMDA-concentration. Moreover, we show that in the absence of microglia the differential changes in neuronal vulnerability between CXCR3(-/-) and wild type OHSCs are fully abrogated and therefore a prominent role for microglia in this process is suggested. Taken together, our results identify a region-specific role for CXCL10/CXCR3 signaling in neuron-glia and glia-glia interactions under pathological conditions. (C) 2010 Wiley-Liss, Inc.

KW - chemokines

KW - excitotoxicity

KW - astrocyte

KW - microglia

KW - liposomic clodronate

KW - CENTRAL-NERVOUS-SYSTEM

KW - EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS

KW - CHEMOKINE RECEPTOR CXCR3

KW - MULTIPLE-SCLEROSIS

KW - SLICE CULTURES

KW - IFN-GAMMA

KW - MICROGLIAL CELLS

KW - INDUCIBLE PROTEIN-10

KW - MESSENGER-RNA

KW - GLUTAMATE NEUROTOXICITY

U2 - 10.1002/hipo.20742

DO - 10.1002/hipo.20742

M3 - Article

VL - 21

SP - 220

EP - 232

JO - Hippocampus

JF - Hippocampus

SN - 1050-9631

IS - 2

ER -

ID: 5269128