Publication

Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

van Montfort, R., Pijning, T., Kalk, K., Schuurman-Wolters, G. K., Reizer, J., Safer Jr., M. H., Robillard, G. & Dijkstra, B. W., 17-Jun-1994, In : Journal of Molecular Biology. 239, 4, p. 588-590 3 p.

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

APA

van Montfort, R., Pijning, T., Kalk, K., Schuurman-Wolters, G. K., Reizer, J., Safer Jr., M. H., Robillard, G., & Dijkstra, B. W. (1994). Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli. Journal of Molecular Biology, 239(4), 588-590. https://doi.org/10.1006/jmbi.1994.1399

Author

van Montfort, Robert ; Pijning, Tjaard ; Kalk, Kornelis ; Schuurman-Wolters, Gea K. ; Reizer, Jonathan ; Safer Jr., Milton H. ; Robillard, George ; Dijkstra, Bauke W. / Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli. In: Journal of Molecular Biology. 1994 ; Vol. 239, No. 4. pp. 588-590.

Harvard

van Montfort, R, Pijning, T, Kalk, K, Schuurman-Wolters, GK, Reizer, J, Safer Jr., MH, Robillard, G & Dijkstra, BW 1994, 'Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli', Journal of Molecular Biology, vol. 239, no. 4, pp. 588-590. https://doi.org/10.1006/jmbi.1994.1399

Standard

Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli. / van Montfort, Robert; Pijning, Tjaard; Kalk, Kornelis; Schuurman-Wolters, Gea K.; Reizer, Jonathan; Safer Jr., Milton H.; Robillard, George; Dijkstra, Bauke W.

In: Journal of Molecular Biology, Vol. 239, No. 4, 17.06.1994, p. 588-590.

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

Vancouver

van Montfort R, Pijning T, Kalk K, Schuurman-Wolters GK, Reizer J, Safer Jr. MH et al. Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli. Journal of Molecular Biology. 1994 Jun 17;239(4):588-590. https://doi.org/10.1006/jmbi.1994.1399


BibTeX

@article{5148d91381ec448e9cb04a082fd340b6,
title = "Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli",
abstract = "Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 {\AA} resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 {\AA}, b = 31.7 {\AA}, c = 60.0 {\AA} and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.",
keywords = "CRYSTALLIZATION, X-RAY, CELLOBIOSE TRANSPORT, PERMEASE, PHOSPHOTRANSFERASE, PHOSPHOENOLPYRUVATE, PHOSPHORYLATION, TRANSPORT, PROTEINS, BACTERIA, DETECTOR",
author = "{van Montfort}, Robert and Tjaard Pijning and Kornelis Kalk and Schuurman-Wolters, {Gea K.} and Jonathan Reizer and {Safer Jr.}, {Milton H.} and George Robillard and Dijkstra, {Bauke W.}",
note = "Relation: https://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",
year = "1994",
month = jun,
day = "17",
doi = "10.1006/jmbi.1994.1399",
language = "English",
volume = "239",
pages = "588--590",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press",
number = "4",

}

RIS

TY - JOUR

T1 - Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

AU - van Montfort, Robert

AU - Pijning, Tjaard

AU - Kalk, Kornelis

AU - Schuurman-Wolters, Gea K.

AU - Reizer, Jonathan

AU - Safer Jr., Milton H.

AU - Robillard, George

AU - Dijkstra, Bauke W.

N1 - Relation: https://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1994/6/17

Y1 - 1994/6/17

N2 - Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.

AB - Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.

KW - CRYSTALLIZATION

KW - X-RAY

KW - CELLOBIOSE TRANSPORT

KW - PERMEASE

KW - PHOSPHOTRANSFERASE

KW - PHOSPHOENOLPYRUVATE

KW - PHOSPHORYLATION

KW - TRANSPORT

KW - PROTEINS

KW - BACTERIA

KW - DETECTOR

U2 - 10.1006/jmbi.1994.1399

DO - 10.1006/jmbi.1994.1399

M3 - Comment/Letter to the editor

VL - 239

SP - 588

EP - 590

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -

ID: 3290047