Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia colivan Montfort, R., Pijning, T., Kalk, K., Schuurman-Wolters, G. K., Reizer, J., Safer Jr., M. H., Robillard, G. & Dijkstra, B. W., 17-Jun-1994, In : Journal of Molecular Biology. 239, 4, p. 588-590 3 p.
Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review
Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.
- CRYSTALLIZATION, X-RAY, CELLOBIOSE TRANSPORT, PERMEASE, PHOSPHOTRANSFERASE, PHOSPHOENOLPYRUVATE, PHOSPHORYLATION, TRANSPORT, PROTEINS, BACTERIA, DETECTOR