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Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

van Montfort, R., Pijning, T., Kalk, K., Schuurman-Wolters, G. K., Reizer, J., Safer Jr., M. H., Robillard, G. & Dijkstra, B. W., 17-Jun-1994, In : Journal of Molecular Biology. 239, 4, p. 588-590 3 p.

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DOI

  • Robert van Montfort
  • Tjaard Pijning
  • Kornelis Kalk
  • Gea K. Schuurman-Wolters
  • Jonathan Reizer
  • Milton H. Safer Jr.
  • George Robillard
  • Bauke W. Dijkstra
Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.
Original languageEnglish
Pages (from-to)588-590
Number of pages3
JournalJournal of Molecular Biology
Volume239
Issue number4
Publication statusPublished - 17-Jun-1994

    Keywords

  • CRYSTALLIZATION, X-RAY, CELLOBIOSE TRANSPORT, PERMEASE, PHOSPHOTRANSFERASE, PHOSPHOENOLPYRUVATE, PHOSPHORYLATION, TRANSPORT, PROTEINS, BACTERIA, DETECTOR

ID: 3290047