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Criteria for Viability Assessment of Discarded Human Donor Livers during Ex Vivo Normothermic Machine Perfusion

Sutton, M. E., op den Dries, S., Karimian, N., Weeder, P. D., de Boer, M. T., Wiersema-Buist, J., Gouw, A. S. H., Leuvenink, H. G. D., Lisman, T. & Porte, R. J., 4-Nov-2014, In : PLoS ONE. 9, 11, 10 p., e110642.

Research output: Contribution to journalArticleAcademicpeer-review

Although normothermic machine perfusion of donor livers may allow assessment of graft viability prior to transplantation, there are currently no data on what would be a good parameter of graft viability. To determine whether bile production is a suitable biomarker that can be used to discriminate viable from non-viable livers we have studied functional performance as well as biochemical and histological evidence of hepatobiliary injury during ex vivo normothermic machine perfusion of human donor livers. After a median duration of cold storage of 6.5 h, twelve extended criteria human donor livers that were declined for transplantation were ex vivo perfused for 6 h at 37 degrees C with an oxygenated solution based on red blood cells and plasma, using pressure controlled pulsatile perfusion of the hepatic artery and continuous portal perfusion. During perfusion, two patterns of bile flow were identified: (1) steadily increasing bile production, resulting in a cumulative output of >= 30 g after 6 h (high bile output group), and (2) a cumulative bile production

Original languageEnglish
Article numbere110642
Number of pages10
JournalPLoS ONE
Volume9
Issue number11
Publication statusPublished - 4-Nov-2014

    Keywords

  • BILE-SALT TOXICITY, BILIARY STRICTURES, COLD-STORAGE, TRANSPLANTATION, PRESERVATION, INJURY, DONATION, GRAFTS, DEATH, DUCTS

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