Publication

Cow's milk allergy in Dutch children: an epigenetic pilot survey

Petrus, N. C. M., Henneman, P., Venema, A., Mul, A., van Sinderen, F., Haagmans, M., Mook, O., Hennekam, R. C., Sprikkelman, A. B. & Mannens, M., 4-May-2016, In : Clinical and translational allergy. 6, 15 p., 16.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Petrus, N. C. M., Henneman, P., Venema, A., Mul, A., van Sinderen, F., Haagmans, M., Mook, O., Hennekam, R. C., Sprikkelman, A. B., & Mannens, M. (2016). Cow's milk allergy in Dutch children: an epigenetic pilot survey. Clinical and translational allergy, 6, [16]. https://doi.org/10.1186/s13601-016-0105-z

Author

Petrus, Nicole C. M. ; Henneman, Peter ; Venema, Andrea ; Mul, Adri ; van Sinderen, Femke ; Haagmans, Martin ; Mook, Olaf ; Hennekam, Raoul C. ; Sprikkelman, Aline B. ; Mannens, Marcel. / Cow's milk allergy in Dutch children : an epigenetic pilot survey. In: Clinical and translational allergy. 2016 ; Vol. 6.

Harvard

Petrus, NCM, Henneman, P, Venema, A, Mul, A, van Sinderen, F, Haagmans, M, Mook, O, Hennekam, RC, Sprikkelman, AB & Mannens, M 2016, 'Cow's milk allergy in Dutch children: an epigenetic pilot survey', Clinical and translational allergy, vol. 6, 16. https://doi.org/10.1186/s13601-016-0105-z

Standard

Cow's milk allergy in Dutch children : an epigenetic pilot survey. / Petrus, Nicole C. M.; Henneman, Peter; Venema, Andrea; Mul, Adri; van Sinderen, Femke; Haagmans, Martin; Mook, Olaf; Hennekam, Raoul C.; Sprikkelman, Aline B.; Mannens, Marcel.

In: Clinical and translational allergy, Vol. 6, 16, 04.05.2016.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Petrus NCM, Henneman P, Venema A, Mul A, van Sinderen F, Haagmans M et al. Cow's milk allergy in Dutch children: an epigenetic pilot survey. Clinical and translational allergy. 2016 May 4;6. 16. https://doi.org/10.1186/s13601-016-0105-z


BibTeX

@article{4020d2eaa1144181b181a18c5a546342,
title = "Cow's milk allergy in Dutch children: an epigenetic pilot survey",
abstract = "Background: Cow's milk allergy (CMA) is a common disease in infancy. Early environmental factors are likely to contribute to CMA. It is known that epigenetic gene regulation can be altered by environmental factors. We have set up a proof of concept study, aiming to detect epigenetic associations specific with CMA.Methods: We studied children from the Dutch EuroPrevall birth cohort study (N = 20 CMA, N = 23 controls, N = 10 tolerant boys), age and gender matched. CMA was challenge proven. Bisulfite converted DNA (blood) was analyzed using the 450K infinium DNA-methylation array. Four groups (combined, girls, boys and tolerant boys) were analysed between CMA and controls. Statistical analysis and pathway-analysis were performed in {"}R{"} using IMA, Minfi and the global-test package. Differentially methylated regions in DHX58, ZNF281, EIF42A and HTRA2 genes were validated by quantitative amplicon sequencing (ROCHE 454 (R)).Results: General hypermethylation was found in the CMA group compared to control children, while this effect was absent in the tolerant group. Methylation differences were, among others, found in regions of DHX58, ZNF281, EIF42A and HTRA2 genes. Several of these genes are known to be involved in immunological pathways and associated with other allergies.Conclusion: We show that epigenetic associations are involved in CMA. Although, the statistical power of our study is limited and our sample was based on whole blood, we were still able to detect feasible loci and pathways. Therefore our findings might contribute to future diagnostic or therapeutic interventions for specific CMA. Further studies have to confirm the findings of our study.",
keywords = "Cow's milk allergy, Epigenetics, Food allergy, Gender differences, Tolerant, EUROPREVALL BIRTH COHORT, FOOD ALLERGY, DNA METHYLATION, CLINICAL-COURSE, NATURAL COURSE, EARLY-LIFE, SENSITIZATION, CHILDHOOD, DISEASES, IMMUNITY",
author = "Petrus, {Nicole C. M.} and Peter Henneman and Andrea Venema and Adri Mul and {van Sinderen}, Femke and Martin Haagmans and Olaf Mook and Hennekam, {Raoul C.} and Sprikkelman, {Aline B.} and Marcel Mannens",
year = "2016",
month = may,
day = "4",
doi = "10.1186/s13601-016-0105-z",
language = "English",
volume = "6",
journal = "Clinical and translational allergy",
issn = "2045-7022",
publisher = "BMC",

}

RIS

TY - JOUR

T1 - Cow's milk allergy in Dutch children

T2 - an epigenetic pilot survey

AU - Petrus, Nicole C. M.

AU - Henneman, Peter

AU - Venema, Andrea

AU - Mul, Adri

AU - van Sinderen, Femke

AU - Haagmans, Martin

AU - Mook, Olaf

AU - Hennekam, Raoul C.

AU - Sprikkelman, Aline B.

AU - Mannens, Marcel

PY - 2016/5/4

Y1 - 2016/5/4

N2 - Background: Cow's milk allergy (CMA) is a common disease in infancy. Early environmental factors are likely to contribute to CMA. It is known that epigenetic gene regulation can be altered by environmental factors. We have set up a proof of concept study, aiming to detect epigenetic associations specific with CMA.Methods: We studied children from the Dutch EuroPrevall birth cohort study (N = 20 CMA, N = 23 controls, N = 10 tolerant boys), age and gender matched. CMA was challenge proven. Bisulfite converted DNA (blood) was analyzed using the 450K infinium DNA-methylation array. Four groups (combined, girls, boys and tolerant boys) were analysed between CMA and controls. Statistical analysis and pathway-analysis were performed in "R" using IMA, Minfi and the global-test package. Differentially methylated regions in DHX58, ZNF281, EIF42A and HTRA2 genes were validated by quantitative amplicon sequencing (ROCHE 454 (R)).Results: General hypermethylation was found in the CMA group compared to control children, while this effect was absent in the tolerant group. Methylation differences were, among others, found in regions of DHX58, ZNF281, EIF42A and HTRA2 genes. Several of these genes are known to be involved in immunological pathways and associated with other allergies.Conclusion: We show that epigenetic associations are involved in CMA. Although, the statistical power of our study is limited and our sample was based on whole blood, we were still able to detect feasible loci and pathways. Therefore our findings might contribute to future diagnostic or therapeutic interventions for specific CMA. Further studies have to confirm the findings of our study.

AB - Background: Cow's milk allergy (CMA) is a common disease in infancy. Early environmental factors are likely to contribute to CMA. It is known that epigenetic gene regulation can be altered by environmental factors. We have set up a proof of concept study, aiming to detect epigenetic associations specific with CMA.Methods: We studied children from the Dutch EuroPrevall birth cohort study (N = 20 CMA, N = 23 controls, N = 10 tolerant boys), age and gender matched. CMA was challenge proven. Bisulfite converted DNA (blood) was analyzed using the 450K infinium DNA-methylation array. Four groups (combined, girls, boys and tolerant boys) were analysed between CMA and controls. Statistical analysis and pathway-analysis were performed in "R" using IMA, Minfi and the global-test package. Differentially methylated regions in DHX58, ZNF281, EIF42A and HTRA2 genes were validated by quantitative amplicon sequencing (ROCHE 454 (R)).Results: General hypermethylation was found in the CMA group compared to control children, while this effect was absent in the tolerant group. Methylation differences were, among others, found in regions of DHX58, ZNF281, EIF42A and HTRA2 genes. Several of these genes are known to be involved in immunological pathways and associated with other allergies.Conclusion: We show that epigenetic associations are involved in CMA. Although, the statistical power of our study is limited and our sample was based on whole blood, we were still able to detect feasible loci and pathways. Therefore our findings might contribute to future diagnostic or therapeutic interventions for specific CMA. Further studies have to confirm the findings of our study.

KW - Cow's milk allergy

KW - Epigenetics

KW - Food allergy

KW - Gender differences

KW - Tolerant

KW - EUROPREVALL BIRTH COHORT

KW - FOOD ALLERGY

KW - DNA METHYLATION

KW - CLINICAL-COURSE

KW - NATURAL COURSE

KW - EARLY-LIFE

KW - SENSITIZATION

KW - CHILDHOOD

KW - DISEASES

KW - IMMUNITY

U2 - 10.1186/s13601-016-0105-z

DO - 10.1186/s13601-016-0105-z

M3 - Article

VL - 6

JO - Clinical and translational allergy

JF - Clinical and translational allergy

SN - 2045-7022

M1 - 16

ER -

ID: 71332518