Publication

Counting stem cells: methodological constraints

Bystrykh, L. V., Verovskaya, E., Zwart, E., Broekhuis, M. & de Haan, G., Jun-2012, In : Nature Methods. 9, 6, p. 567-574 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Bystrykh, L. V., Verovskaya, E., Zwart, E., Broekhuis, M., & de Haan, G. (2012). Counting stem cells: methodological constraints. Nature Methods, 9(6), 567-574. https://doi.org/10.1038/NMETH.2043

Author

Bystrykh, Leonid V. ; Verovskaya, Evgenia ; Zwart, Erik ; Broekhuis, Mathilde ; de Haan, Gerald. / Counting stem cells : methodological constraints. In: Nature Methods. 2012 ; Vol. 9, No. 6. pp. 567-574.

Harvard

Bystrykh, LV, Verovskaya, E, Zwart, E, Broekhuis, M & de Haan, G 2012, 'Counting stem cells: methodological constraints', Nature Methods, vol. 9, no. 6, pp. 567-574. https://doi.org/10.1038/NMETH.2043

Standard

Counting stem cells : methodological constraints. / Bystrykh, Leonid V.; Verovskaya, Evgenia; Zwart, Erik; Broekhuis, Mathilde; de Haan, Gerald.

In: Nature Methods, Vol. 9, No. 6, 06.2012, p. 567-574.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Bystrykh LV, Verovskaya E, Zwart E, Broekhuis M, de Haan G. Counting stem cells: methodological constraints. Nature Methods. 2012 Jun;9(6):567-574. https://doi.org/10.1038/NMETH.2043


BibTeX

@article{7f17fe78236d4cc8985308cb5de77971,
title = "Counting stem cells: methodological constraints",
abstract = "The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.",
keywords = "SCID-X1 GENE-THERAPY, ADENOSINE-DEAMINASE DEFICIENCY, INTEGRATION SITE ANALYSIS, VECTOR INTEGRATION, RETROVIRAL INTEGRATION, HEMATOPOIETIC SYSTEM, PERIPHERAL-BLOOD, PROGENITOR CELLS, CD34(+) CELLS, CORD BLOOD",
author = "Bystrykh, {Leonid V.} and Evgenia Verovskaya and Erik Zwart and Mathilde Broekhuis and {de Haan}, Gerald",
year = "2012",
month = "6",
doi = "10.1038/NMETH.2043",
language = "English",
volume = "9",
pages = "567--574",
journal = "Nature Methods",
issn = "1548-7105",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - Counting stem cells

T2 - methodological constraints

AU - Bystrykh, Leonid V.

AU - Verovskaya, Evgenia

AU - Zwart, Erik

AU - Broekhuis, Mathilde

AU - de Haan, Gerald

PY - 2012/6

Y1 - 2012/6

N2 - The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.

AB - The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.

KW - SCID-X1 GENE-THERAPY

KW - ADENOSINE-DEAMINASE DEFICIENCY

KW - INTEGRATION SITE ANALYSIS

KW - VECTOR INTEGRATION

KW - RETROVIRAL INTEGRATION

KW - HEMATOPOIETIC SYSTEM

KW - PERIPHERAL-BLOOD

KW - PROGENITOR CELLS

KW - CD34(+) CELLS

KW - CORD BLOOD

U2 - 10.1038/NMETH.2043

DO - 10.1038/NMETH.2043

M3 - Article

VL - 9

SP - 567

EP - 574

JO - Nature Methods

JF - Nature Methods

SN - 1548-7105

IS - 6

ER -

ID: 5581389