Publication

Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia

Kapanadze, BI., Brodyanskii, VM., Semov, AB., Baranova, AV., Sulimova, GE., Aitova, SS., Udina, IG., Ptitsyna, SN., Salnikova, LE., Chudinov, OS., Borbiev, TE., Kashuba, VV., Gizatullin, R., Zabarovska, N. V., Zabarovsky, ER., Fedorova, LI., Zelenin, AV., Rasool, O., Grander, D., Einhorn, S., vanEverdink, W., van den Berg, A., Buys, C., Corcoran, M., Chapman, RM. & Yankovsky, NK., 1997, In : Molecular biology. 31, 3, p. 431-434 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • BI Kapanadze
  • VM Brodyanskii
  • AB Semov
  • AV Baranova
  • GE Sulimova
  • SS Aitova
  • IG Udina
  • SN Ptitsyna
  • LE Salnikova
  • OS Chudinov
  • TE Borbiev
  • VV Kashuba
  • R Gizatullin
  • [No Value] Zabarovska
  • ER Zabarovsky
  • LI Fedorova
  • AV Zelenin
  • O Rasool
  • D Grander
  • S Einhorn
  • W vanEverdink
  • Anke van den Berg
  • C Buys
  • M Corcoran
  • RM Chapman
  • NK Yankovsky

We constructed a fine physical map of human chromosome 13q14 region between D13S1168 and D13S25 loci consisting of cosmid and cDNA clones. This interval had been shown to be in the center of the genome region frequently lost in a human blood malignancy known as B-cell chronic lymphocytic leukemia (BCLL), Mapping of the genome region is a step in searching for a putative tumor suppressor gene for BCLL. A chr13-specific cosmid library (LA13NC01) was screened with four YAC and seven WI-STS markers belonging to the above 13q14 interval, yielding a cosmid subset of more than 400 clones representing the region. Cosmids between D13S1168 and D13S25 loci were arranged in contigs. Seven different clones were found in cDNA libraries by hybridization screening with YAC ICRF66c1 and two cosmids covering the D13S319 locus, which is the central point for BCLL-associated deletions, The cDNA clones were mapped against the contigous cosmids.

Original languageEnglish
Pages (from-to)431-434
Number of pages4
JournalMolecular biology
Volume31
Issue number3
Publication statusPublished - 1997

    Keywords

  • human chromosome 13, 13q14 region, cosmid map, cDNA map, chronic lymphocytic leukemia, D13S25 LOCUS, LIBRARIES, DELETION, GENE, CONSTRUCTION, BREAKPOINT

ID: 6525582