Publication

Correlated light and electron microscopy: ultrastructure lights up!

de Boer, P., Hoogenboom, J. P. & Giepmans, B. N. G., Jun-2015, In : Nature Methods. 12, 6, p. 503-513 11 p.

Research output: Contribution to journalReview articleAcademicpeer-review

APA

de Boer, P., Hoogenboom, J. P., & Giepmans, B. N. G. (2015). Correlated light and electron microscopy: ultrastructure lights up! Nature Methods, 12(6), 503-513. https://doi.org/10.1038/nmeth.3400

Author

de Boer, Pascal ; Hoogenboom, Jacob P. ; Giepmans, Ben N. G. / Correlated light and electron microscopy : ultrastructure lights up!. In: Nature Methods. 2015 ; Vol. 12, No. 6. pp. 503-513.

Harvard

de Boer, P, Hoogenboom, JP & Giepmans, BNG 2015, 'Correlated light and electron microscopy: ultrastructure lights up!', Nature Methods, vol. 12, no. 6, pp. 503-513. https://doi.org/10.1038/nmeth.3400

Standard

Correlated light and electron microscopy : ultrastructure lights up! / de Boer, Pascal; Hoogenboom, Jacob P.; Giepmans, Ben N. G.

In: Nature Methods, Vol. 12, No. 6, 06.2015, p. 503-513.

Research output: Contribution to journalReview articleAcademicpeer-review

Vancouver

de Boer P, Hoogenboom JP, Giepmans BNG. Correlated light and electron microscopy: ultrastructure lights up! Nature Methods. 2015 Jun;12(6):503-513. https://doi.org/10.1038/nmeth.3400


BibTeX

@article{0307ed02d69643b4b8cbc06daf6cf932,
title = "Correlated light and electron microscopy: ultrastructure lights up!",
abstract = "Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek observed living microorganisms and cells in 1674 using his light microscope. A spectrum of dyes and probes now enable the localization of molecules of interest within living cells by fluorescence microscopy. With electron microscopy (EM), cellular ultrastructure has been revealed. Bridging these two modalities, correlated light microscopy and EM (CLEM) opens new avenues. Studies of protein dynamics with fluorescent proteins (FPs), which leave the investigator 'in the dark' concerning cellular context, can be followed by EM examination. Rare events can be preselected at the light microscopy level before EM analysis. Ongoing development-including of dedicated probes, integrated microscopes, large-scale and three-dimensional EM and super-resolution fluorescence microscopy-now paves the way for broad CLEM implementation in biology.",
keywords = "FLUORESCENCE MICROSCOPY, SUPERRESOLUTION FLUORESCENCE, HIGH-RESOLUTION, CRYOELECTRON TOMOGRAPHY, EUKARYOTIC CELLS, SPATIAL PRECISION, CRYO-FLUORESCENCE, INTEGRATED LIGHT, FIDUCIAL MARKERS, MAMMALIAN-CELLS",
author = "{de Boer}, Pascal and Hoogenboom, {Jacob P.} and Giepmans, {Ben N. G.}",
year = "2015",
month = "6",
doi = "10.1038/nmeth.3400",
language = "English",
volume = "12",
pages = "503--513",
journal = "Nature Methods",
issn = "1548-7105",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - Correlated light and electron microscopy

T2 - ultrastructure lights up!

AU - de Boer, Pascal

AU - Hoogenboom, Jacob P.

AU - Giepmans, Ben N. G.

PY - 2015/6

Y1 - 2015/6

N2 - Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek observed living microorganisms and cells in 1674 using his light microscope. A spectrum of dyes and probes now enable the localization of molecules of interest within living cells by fluorescence microscopy. With electron microscopy (EM), cellular ultrastructure has been revealed. Bridging these two modalities, correlated light microscopy and EM (CLEM) opens new avenues. Studies of protein dynamics with fluorescent proteins (FPs), which leave the investigator 'in the dark' concerning cellular context, can be followed by EM examination. Rare events can be preselected at the light microscopy level before EM analysis. Ongoing development-including of dedicated probes, integrated microscopes, large-scale and three-dimensional EM and super-resolution fluorescence microscopy-now paves the way for broad CLEM implementation in biology.

AB - Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek observed living microorganisms and cells in 1674 using his light microscope. A spectrum of dyes and probes now enable the localization of molecules of interest within living cells by fluorescence microscopy. With electron microscopy (EM), cellular ultrastructure has been revealed. Bridging these two modalities, correlated light microscopy and EM (CLEM) opens new avenues. Studies of protein dynamics with fluorescent proteins (FPs), which leave the investigator 'in the dark' concerning cellular context, can be followed by EM examination. Rare events can be preselected at the light microscopy level before EM analysis. Ongoing development-including of dedicated probes, integrated microscopes, large-scale and three-dimensional EM and super-resolution fluorescence microscopy-now paves the way for broad CLEM implementation in biology.

KW - FLUORESCENCE MICROSCOPY

KW - SUPERRESOLUTION FLUORESCENCE

KW - HIGH-RESOLUTION

KW - CRYOELECTRON TOMOGRAPHY

KW - EUKARYOTIC CELLS

KW - SPATIAL PRECISION

KW - CRYO-FLUORESCENCE

KW - INTEGRATED LIGHT

KW - FIDUCIAL MARKERS

KW - MAMMALIAN-CELLS

U2 - 10.1038/nmeth.3400

DO - 10.1038/nmeth.3400

M3 - Review article

VL - 12

SP - 503

EP - 513

JO - Nature Methods

JF - Nature Methods

SN - 1548-7105

IS - 6

ER -

ID: 20938213