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Control of Propionibacterium acnes by natural antimicrobial substances: Role of the bacteriocin AS-48 and lysozyme

Cebrian, R., Arevalo, S., Rubino, S., Arias-Santiago, S., Rojo, M. D., Montalban-Lopez, M., Martinez-Bueno, M., Valdivia, E. & Maqueda, M., 6-Aug-2018, In : Scientific Reports. 8, 11 p., 11766.

Research output: Contribution to journalArticleAcademicpeer-review

  • Ruben Cebrian
  • Sergio Arevalo
  • Susana Rubino
  • Salvador Arias-Santiago
  • Maria Dolores Rojo
  • Manuel Montalban-Lopez
  • Manuel Martinez-Bueno
  • Eva Valdivia
  • Mercedes Maqueda

We report the high susceptibility of several clinical isolates of Propionibacterium acnes from different sources (skin, bone, wound exudates, abscess or blood contamination) to the head-to-tail cyclized bacteriocin AS-48. This peptide is a feasible candidate for further pharmacological development against this bacterium, due to its physicochemical and biological characteristics, even when it is growing in a biofilm. Thus, the treatment of pre-formed biofilms with AS-48 resulted in a dose-and time-dependent disruption of the biofilm architecture beside the decrease of bacterial viability. Furthermore, we demonstrated the potential of lysozyme to bolster the inhibitory activity of AS-48 against P. acnes, rendering high reductions in the MIC values, even in matrix-growing cultures, according to the results obtained using a range of microscopy and bioassay techniques. The improvement of the activity of AS-48 through its co-formulation with lysozyme may be considered an alternative in the control of P. acnes, especially after proving the absence of cytotoxicity demonstrated by these natural compounds on relevant human skin cell lines. In summary, this study supports that compositions comprising the bacteriocin AS-48 plus lysozyme must be considered as promising candidates for topical applications with medical and pharmaceutical purposes against dermatological diseases such as acne vulgaris.

Original languageEnglish
Article number11766
Number of pages11
JournalScientific Reports
Volume8
Publication statusPublished - 6-Aug-2018

    Keywords

  • GRAM-NEGATIVE BACTERIA, BIOFILM FORMATION, ENTEROCIN AS-48, FOOD PRESERVATION, SKIN, ANTIBIOTICS, INFECTIONS, BIOMATERIALS, OPTIMIZATION, PATHOGENESIS

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