Publication

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Huberts, D. H. E. W., Janssens, G. E., Lee, S. S., Vizcarra, I. A. & Heinemann, M., Aug-2013, In : Journal of visualized experiments : JoVE. 78, 7 p., 50143.

Research output: Contribution to journalArticleAcademicpeer-review

  • Daphne H. E. W. Huberts
  • Georges E. Janssens
  • Sung Sik Lee
  • Ima Avalos Vizcarra
  • Matthias Heinemann

We demonstrate the use of a simple microfluidic setup, in which single budding yeast cells can be tracked throughout their entire lifespan. The microfluidic chip exploits the size difference between mother and daughter cells using an array of micropads. Upon loading, cells are trapped underneath these micropads, because the distance between the micropad and cover glass is similar to the diameter of a yeast cell (3-4 mu m). After the loading procedure, culture medium is continuously flushed through the chip, which not only creates a constant and defined environment throughout the entire experiment, but also flushes out the emerging daughter cells, which are not retained underneath the pads due to their smaller size. The setup retains mother cells so efficiently that in a single experiment up to 50 individual cells can be monitored in a fully automated manner for 5 days or, if necessary, longer. In addition, the excellent optical properties of the chip allow high-resolution imaging of cells during the entire aging process.

Original languageEnglish
Article number50143
Number of pages7
JournalJournal of visualized experiments : JoVE
Issue number78
Publication statusPublished - Aug-2013

    Keywords

  • Bioengineering, Issue 78, Microbiology, Cellular Biology, Molecular Biology, Biochemistry, Biomedical Engineering, Genetics, Anatomy, Physiology, Lifespan analysis, Live-cell imaging, Microfluidics, Aging, Microscopy, Saccharomyces cerevisiae, microfluidic chip, yeast, cell culture, cells, imaging

ID: 12993059