Publication

Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH: Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion

Price, C. E. & Driessen, A. J. M., 5-Feb-2010, In : The Journal of Biological Chemistry. 285, 6, p. 3575-3581 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Price, C. E., & Driessen, A. J. M. (2010). Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH: Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion. The Journal of Biological Chemistry, 285(6), 3575-3581. https://doi.org/10.1074/jbc.M109.051128

Author

Price, Claire E. ; Driessen, Arnold J. M. / Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH : Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion. In: The Journal of Biological Chemistry. 2010 ; Vol. 285, No. 6. pp. 3575-3581.

Harvard

Price, CE & Driessen, AJM 2010, 'Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH: Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion', The Journal of Biological Chemistry, vol. 285, no. 6, pp. 3575-3581. https://doi.org/10.1074/jbc.M109.051128

Standard

Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH : Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion. / Price, Claire E.; Driessen, Arnold J. M.

In: The Journal of Biological Chemistry, Vol. 285, No. 6, 05.02.2010, p. 3575-3581.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Price CE, Driessen AJM. Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH: Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion. The Journal of Biological Chemistry. 2010 Feb 5;285(6):3575-3581. https://doi.org/10.1074/jbc.M109.051128


BibTeX

@article{673147d2168043d299da5f7ef5756710,
title = "Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH: Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion",
abstract = "All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system. Although the range of proteins shown to be dependent on YidC continues to increase, the exact role of YidC in insertion remains enigmatic. Here we show that YidC is essential for the insertion of subunit K of the NADH: ubiquinone oxidoreductase and that the dependence is due to the presence of two conserved glutamate residues in the transmembrane segments of subunit K. The results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.",
keywords = "NADH-UBIQUINONE OXIDOREDUCTASE, ESCHERICHIA-COLI, INNER-MEMBRANE, SACCHAROMYCES-CEREVISIAE, PROTEIN INSERTION, F1FO-ATP SYNTHASE, ATP SYNTHASE, NUCLEAR GENE, COMPLEX-I, BIOGENESIS",
author = "Price, {Claire E.} and Driessen, {Arnold J. M.}",
year = "2010",
month = feb,
day = "5",
doi = "10.1074/jbc.M109.051128",
language = "English",
volume = "285",
pages = "3575--3581",
journal = "The Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC",
number = "6",

}

RIS

TY - JOUR

T1 - Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH

T2 - Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion

AU - Price, Claire E.

AU - Driessen, Arnold J. M.

PY - 2010/2/5

Y1 - 2010/2/5

N2 - All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system. Although the range of proteins shown to be dependent on YidC continues to increase, the exact role of YidC in insertion remains enigmatic. Here we show that YidC is essential for the insertion of subunit K of the NADH: ubiquinone oxidoreductase and that the dependence is due to the presence of two conserved glutamate residues in the transmembrane segments of subunit K. The results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.

AB - All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system. Although the range of proteins shown to be dependent on YidC continues to increase, the exact role of YidC in insertion remains enigmatic. Here we show that YidC is essential for the insertion of subunit K of the NADH: ubiquinone oxidoreductase and that the dependence is due to the presence of two conserved glutamate residues in the transmembrane segments of subunit K. The results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.

KW - NADH-UBIQUINONE OXIDOREDUCTASE

KW - ESCHERICHIA-COLI

KW - INNER-MEMBRANE

KW - SACCHAROMYCES-CEREVISIAE

KW - PROTEIN INSERTION

KW - F1FO-ATP SYNTHASE

KW - ATP SYNTHASE

KW - NUCLEAR GENE

KW - COMPLEX-I

KW - BIOGENESIS

U2 - 10.1074/jbc.M109.051128

DO - 10.1074/jbc.M109.051128

M3 - Article

VL - 285

SP - 3575

EP - 3581

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

SN - 0021-9258

IS - 6

ER -

ID: 5039390