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Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH: Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion

Price, C. E. & Driessen, A. J. M., 5-Feb-2010, In : The Journal of Biological Chemistry. 285, 6, p. 3575-3581 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system. Although the range of proteins shown to be dependent on YidC continues to increase, the exact role of YidC in insertion remains enigmatic. Here we show that YidC is essential for the insertion of subunit K of the NADH: ubiquinone oxidoreductase and that the dependence is due to the presence of two conserved glutamate residues in the transmembrane segments of subunit K. The results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.

Original languageEnglish
Pages (from-to)3575-3581
Number of pages7
JournalThe Journal of Biological Chemistry
Volume285
Issue number6
Publication statusPublished - 5-Feb-2010

    Keywords

  • NADH-UBIQUINONE OXIDOREDUCTASE, ESCHERICHIA-COLI, INNER-MEMBRANE, SACCHAROMYCES-CEREVISIAE, PROTEIN INSERTION, F1FO-ATP SYNTHASE, ATP SYNTHASE, NUCLEAR GENE, COMPLEX-I, BIOGENESIS

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