Publication

Conserved Citrullinating Exoenzymes in Porphyromonas Species

Gabarrini, G., Chlebowicz, M. A., Vega Quiroz, M. E., Veloo, A. C. M., Rossen, J. W. A., Harmsen, H. J. M., Laine, M. L., van Dijl, J. M. & van Winkelhoff, A. J., May-2018, In : Journal of Dental Research. 97, 5, p. 556-562 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Conserved Citrullinating Exoenzymes in Porphyromonas Species

    Final publisher's version, 774 KB, PDF document

DOI

Porphyromonas gingivalis is one of the major oral pathogens implicated in the widespread inflammatory disorder periodontitis. Moreover, in recent years, P. gingivalis has been associated with the autoimmune disease rheumatoid arthritis. The peptidylarginine deiminase enzyme of P. gingivalis (PPAD) is a major virulence factor that catalyzes the citrullination of both bacterial and host proteins, potentially contributing to production of anticitrullinated protein antibodies. Considering that these antibodies are very specific for rheumatoid arthritis, PPAD appears to be a link between P. gingivalis, periodontitis, and the autoimmune disorder rheumatoid arthritis. PPAD was thus far considered unique among prokaryotes, with P. gingivalis being the only bacterium known to produce and secrete it. To challenge this hypothesis, we investigated the possible secretion of PPAD by 11 previously collected Porphyromonas isolates from a dog, 2 sheep, 3 cats, 4 monkeys, and a jaguar with periodontitis. Our analyses uncovered the presence of secreted PPAD homologues in 8 isolates that were identified as Porphyromonas gulae (from a dog, monkeys, and cats) and Porphyromonas loveana (from sheep). In all 3 PPAD-producing Porphyromonas species, the dominant form of the secreted PPAD was associated with outer membrane vesicles, while a minor fraction was soluble. Our results prove for the first time that the citrullinating PPAD exoenzyme is not unique to only 1 prokaryotic species. Instead, we show that PPAD is produced by at least 2 other oral pathogens.

Original languageEnglish
Pages (from-to)556-562
Number of pages7
JournalJournal of Dental Research
Volume97
Issue number5
Early online date3-Jan-2018
Publication statusPublished - May-2018

    Keywords

  • periodontal disease(s), periodontitis, microbiology, PPAD, citrullination, OMVs, OUTER-MEMBRANE VESICLES, BROKEN MOUTH PERIODONTITIS, RHEUMATOID-ARTHRITIS, PEPTIDYLARGININE DEIMINASE, AUSTRALIAN MARSUPIALS, CANINE ARTHRITIS, ORAL-CAVITY, GINGIVALIS, DISEASE, PROTEINS

View graph of relations

ID: 52681266