Publication

Conformational Dynamics of the Plug Domain of the SecYEG Protein-conducting Channel

Lycklama a Nijeholt, J. A., Wu, Z. C. & Driessen, A. J. M., 23-Dec-2011, In : The Journal of Biological Chemistry. 286, 51, p. 43881-43890 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

The central pore of the SecYEG preprotein-conducting channel is closed at the periplasmic face of the membrane by a plug domain. To study its conformational dynamics, the plug was labeled site-specifically with an environment-sensitive fluorophore. In the presence of a stable preprotein translocation intermediate, the SecY plug showed an enhanced solvent exposure consistent with a displacement from the hydrophobic central pore region. In contrast, binding and insertion of a ribosome-bound nascent membrane protein did not alter the plug conformation. These data indicate different plug dynamics depending on the ligand bound state of the SecYEG channel.

Original languageEnglish
Pages (from-to)43881-43890
Number of pages10
JournalThe Journal of Biological Chemistry
Volume286
Issue number51
Publication statusPublished - 23-Dec-2011

    Keywords

  • NASCENT CHAIN COMPLEXES, COLI MEMBRANE-VESICLES, ESCHERICHIA-COLI, TRANSLOCATION CHANNEL, IN-VIVO, SIGNAL SEQUENCE, CROSS-LINKING, LATERAL GATE, SECA PROTEIN, RIBOSOME

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