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Complex Analysis of Fluorescence Intensity Fluctuations of Molecular Compounds

Yatskou, M. M., Skakun, V. V., Nederveen-Schippers, L., Kortholt, A. & Apanasovich, V. V., 1-Sep-2020, In : Journal of applied spectroscopy. 87, 4, p. 685-692 8 p.

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  • Complex Analysis of Fluorescence Intensity Fluctuations of Molecular Compounds

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DOI

  • M. M. Yatskou
  • V. V. Skakun
  • L. Nederveen-Schippers
  • A. Kortholt
  • V. V. Apanasovich

A method is proposed for the complex analysis of fluctuations in the fluorescence intensity of molecular compounds, which allows determining the structural composition of protein oligomers. The idea of the method is to analyze the photon counting histograms of experimental measurements using principal component analysis to assess the presence of oligomeric compounds, and to perform hierarchical cluster analysis, to determine the data classes corresponding to various molecular compounds, followed by selecting cluster medoids to determine the oligomeric composition of protein complexes. The efficiency of the analysis algorithms developed within the framework of the proposed method was confirmed on simulated and experimental photon counting histograms of the measured fluorescence intensity fluctuations of monomeric and dimeric forms of green-fluorescent protein (GFP).

Original languageEnglish
Pages (from-to)685-692
Number of pages8
JournalJournal of applied spectroscopy
Volume87
Issue number4
Publication statusPublished - 1-Sep-2020

    Keywords

  • fluorescence intensity fluctuation, photon counting histogram, molecular compounds, protein oligomers, data mining, principal component analysis, hierarchical cluster analysis, green-fluorescent protein (GFP)

ID: 135098558