Publication

Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways

Krooneman, J., Moore, ERB., van Velzen, JCL., Prins, RA., Forney, LJ. & GOttschal, JC., Jul-1998, In : FEMS Microbial Ecology. 26, 3, p. 171-179 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Krooneman, J., Moore, ERB., van Velzen, JCL., Prins, RA., Forney, LJ., & GOttschal, JC. (1998). Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways. FEMS Microbial Ecology, 26(3), 171-179.

Author

Krooneman, J ; Moore, ERB ; van Velzen, JCL ; Prins, RA ; Forney, LJ ; GOttschal, JC. / Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways. In: FEMS Microbial Ecology. 1998 ; Vol. 26, No. 3. pp. 171-179.

Harvard

Krooneman, J, Moore, ERB, van Velzen, JCL, Prins, RA, Forney, LJ & GOttschal, JC 1998, 'Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways', FEMS Microbial Ecology, vol. 26, no. 3, pp. 171-179.

Standard

Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways. / Krooneman, J; Moore, ERB; van Velzen, JCL; Prins, RA; Forney, LJ; GOttschal, JC.

In: FEMS Microbial Ecology, Vol. 26, No. 3, 07.1998, p. 171-179.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Krooneman J, Moore ERB, van Velzen JCL, Prins RA, Forney LJ, GOttschal JC. Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways. FEMS Microbial Ecology. 1998 Jul;26(3):171-179.


BibTeX

@article{9601f8c56b9040038ecd4f3b08e18076,
title = "Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways",
abstract = "In nature a significant part of the microbial activity is concentrated at or near oxic/anoxic interfaces, where oxygen concentrations are often low. Bacteria possessing different kinetic characteristics for oxygen and employing distinct metabolic pathways for the degradation of (halo)aromatic substrates for which oxygen is needed as co-substrate may have to compete with each other in such environments. In this study the competitiveness of Pseudomonas sp. strain A3 relative to Alcaligenes sp. strain L6 was tested in batch and in continuous cultures. While both of these strains are able to metabolise 3-chlorobenzoate (3CBA), the former was isolated under air saturating conditions and employs the catechol pathway, whereas the latter was isolated under reduced partial pressures of oxygen and was capable of metabolising 3CBA via the gentisate pathway. Competition experiments in batch culture resulted in pure cultures of Pseudomonas sp. strain A3 under air saturating conditions. However, if reduced partial pressures of oxygen (2{\%}) were used, Alcaligenes sp. strain L6 remained present in substantial numbers after three transfers. Continuous culture experiments demonstrated that Alcaligenes sp. strain L6 was able to outcompete Pseudomonas sp. strain A3 under oxygen- as well as under carbon-limiting conditions as long as the dilution rate remained below 0.136 h(-1) (low oxygen) and below 0.178 h(-1) (high oxygen). These results support the hypothesis that organisms metabolising chlorobenzoate via the gentisate pathway may play a significant role in natural ecosystems where xenobiotic compounds and naturally produced aromatics occur at very low concentrations and in combination with limiting oxygen tensions. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
keywords = "kinetic characteristics, limiting conditions, Pseudomonas, Alcaligenes, HYPOXIC ENVIRONMENTS, BIODEGRADATION, ACID, MINERALIZATION, PSEUDOMONAD, KINETICS",
author = "J Krooneman and ERB Moore and {van Velzen}, JCL and RA Prins and LJ Forney and JC GOttschal",
year = "1998",
month = "7",
language = "English",
volume = "26",
pages = "171--179",
journal = "FEMS Microbial Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Competition for oxygen and 3-chlorobenzoate between two aerobic bacteria using different degradation pathways

AU - Krooneman, J

AU - Moore, ERB

AU - van Velzen, JCL

AU - Prins, RA

AU - Forney, LJ

AU - GOttschal, JC

PY - 1998/7

Y1 - 1998/7

N2 - In nature a significant part of the microbial activity is concentrated at or near oxic/anoxic interfaces, where oxygen concentrations are often low. Bacteria possessing different kinetic characteristics for oxygen and employing distinct metabolic pathways for the degradation of (halo)aromatic substrates for which oxygen is needed as co-substrate may have to compete with each other in such environments. In this study the competitiveness of Pseudomonas sp. strain A3 relative to Alcaligenes sp. strain L6 was tested in batch and in continuous cultures. While both of these strains are able to metabolise 3-chlorobenzoate (3CBA), the former was isolated under air saturating conditions and employs the catechol pathway, whereas the latter was isolated under reduced partial pressures of oxygen and was capable of metabolising 3CBA via the gentisate pathway. Competition experiments in batch culture resulted in pure cultures of Pseudomonas sp. strain A3 under air saturating conditions. However, if reduced partial pressures of oxygen (2%) were used, Alcaligenes sp. strain L6 remained present in substantial numbers after three transfers. Continuous culture experiments demonstrated that Alcaligenes sp. strain L6 was able to outcompete Pseudomonas sp. strain A3 under oxygen- as well as under carbon-limiting conditions as long as the dilution rate remained below 0.136 h(-1) (low oxygen) and below 0.178 h(-1) (high oxygen). These results support the hypothesis that organisms metabolising chlorobenzoate via the gentisate pathway may play a significant role in natural ecosystems where xenobiotic compounds and naturally produced aromatics occur at very low concentrations and in combination with limiting oxygen tensions. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - In nature a significant part of the microbial activity is concentrated at or near oxic/anoxic interfaces, where oxygen concentrations are often low. Bacteria possessing different kinetic characteristics for oxygen and employing distinct metabolic pathways for the degradation of (halo)aromatic substrates for which oxygen is needed as co-substrate may have to compete with each other in such environments. In this study the competitiveness of Pseudomonas sp. strain A3 relative to Alcaligenes sp. strain L6 was tested in batch and in continuous cultures. While both of these strains are able to metabolise 3-chlorobenzoate (3CBA), the former was isolated under air saturating conditions and employs the catechol pathway, whereas the latter was isolated under reduced partial pressures of oxygen and was capable of metabolising 3CBA via the gentisate pathway. Competition experiments in batch culture resulted in pure cultures of Pseudomonas sp. strain A3 under air saturating conditions. However, if reduced partial pressures of oxygen (2%) were used, Alcaligenes sp. strain L6 remained present in substantial numbers after three transfers. Continuous culture experiments demonstrated that Alcaligenes sp. strain L6 was able to outcompete Pseudomonas sp. strain A3 under oxygen- as well as under carbon-limiting conditions as long as the dilution rate remained below 0.136 h(-1) (low oxygen) and below 0.178 h(-1) (high oxygen). These results support the hypothesis that organisms metabolising chlorobenzoate via the gentisate pathway may play a significant role in natural ecosystems where xenobiotic compounds and naturally produced aromatics occur at very low concentrations and in combination with limiting oxygen tensions. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - kinetic characteristics

KW - limiting conditions

KW - Pseudomonas

KW - Alcaligenes

KW - HYPOXIC ENVIRONMENTS

KW - BIODEGRADATION

KW - ACID

KW - MINERALIZATION

KW - PSEUDOMONAD

KW - KINETICS

M3 - Article

VL - 26

SP - 171

EP - 179

JO - FEMS Microbial Ecology

JF - FEMS Microbial Ecology

SN - 0168-6496

IS - 3

ER -

ID: 3733387