Publication

Combined implantation of CD34+and CD14+cells increases neovascularization through amplified paracrine signalling

Krenning, G., van der Strate, B. W. A., Schipper, M., Brouwer, L. A., Fernandes, B. C. A., van Luyn, M. J. A. & Harmsen, M. C., Feb-2013, In : Journal of tissue engineering and regenerative medicine. 7, 2, p. 118-128 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

Cell therapy strategies that use adult peripheral blood-derived CD34+ progenitor cells are hampered by low cell numbers and the infrequent cellular incorporation into the neovasculature. Hence, the use of CD34+ cells to treat ischaemic diseases is under debate. Interaction between CD34+ cells and CD14+ cells results in superior endothelial differentiation of CD14+ cells in vitro, indicating that cell therapy approaches utilizing both CD34+ and CD14+ cells may be advantageous in therapeutic neovascularization. Here, human CD34+ and CD14+ cells were isolated from adult peripheral blood and implanted subcutaneously into nude mice, using matrigel as the carrier. Combined implantation of human CD34+ and CD14+ cells resulted in superior neovascularization, compared to either cell type alone, albeit incorporation of human cells into the murine vasculature was not observed. Human CD34+ and CD14+ cells produced and secreted a pentad of pro-angiogenic mediators, such as HGF, MCP-1 and IL-8, bFGF and VEGFa in monoculture. The production and secretion of pro-angiogenic mediators by CD14+ cells was highly amplified upon incubation with conditioned medium from CD34+ cells. In vivo, neovascularization of matrigel implants did not rely on the endothelial differentiation and incorporation of CD34+ or CD14+ cells, but depended on the paracrine effects of IL-8, MCP-1, HGF, bFGF and VEGFa secreted by implanted cells. Administration of this growth factor/cytokine pentad using matrigel as a carrier results in cell recruitment and microvessel formation equal to progenitor cell-induced neovascularization. These data provide new insights on neovascularization by cell therapy and may contribute to new strategies for the treatment of ischaemic diseases. Copyright (c) 2011 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)118-128
Number of pages11
JournalJournal of tissue engineering and regenerative medicine
Volume7
Issue number2
Publication statusPublished - Feb-2013

    Keywords

  • neovascularization, CD34, CD14, HGF, IL-8, MCP-1, cell therapy, ischaemia, ENDOTHELIAL PROGENITOR CELLS, GROWTH-FACTOR, ANGIOGENESIS, CD34(+), TRANSPLANTATION, DIFFERENTIATION, INFLAMMATION, TRIAL, VIVO

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