Publication

Cloning and characterization of an aromatic amino acid and leucine permease of Penicillium chrysogenum

Trip, H., Evers, M. E., Konings, W. N. & Driessen, A. J. M., 2002, In : Biochimica et Biophysica Acta-Biomembranes. 1565, 1, p. 73 - 80 8 p.

Research output: Contribution to journalArticleAcademic

Copy link to clipboard

Documents

DOI

The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on conserved regions of fungal amino acid permeases. The cDNA clone was used for expression of the permease in Saccharomyces cerevisiae M4054, which is defective in the general amino acid permease Gap1. Upon overexpression, an increase in the uptake of L-tyrosine, L-phenylalanine, L-tryptophan and L-leucine was observed. Further competition experiments indicate that ArlP recognizes neutral and aromatic amino acids with an unbranched β-carbon atom.
Original languageEnglish
Pages (from-to)73 - 80
Number of pages8
JournalBiochimica et Biophysica Acta-Biomembranes
Volume1565
Issue number1
Publication statusPublished - 2002

    Keywords

  • Amino acid uptake, Saccharomyces cerevisiae, Gene expression, Penicillium chrysogenum, Amino acid permease

Download statistics

No data available

ID: 1216810