Cloning and characterization of an aromatic amino acid and leucine permease of Penicillium chrysogenumTrip, H., Evers, M. E., Konings, W. N. & Driessen, A. J. M., 2002, In : Biochimica et Biophysica Acta-Biomembranes. 1565, 1, p. 73 - 80 8 p.
Research output: Contribution to journal › Article › Academic
The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on conserved regions of fungal amino acid permeases. The cDNA clone was used for expression of the permease in Saccharomyces cerevisiae M4054, which is defective in the general amino acid permease Gap1. Upon overexpression, an increase in the uptake of L-tyrosine, L-phenylalanine, L-tryptophan and L-leucine was observed. Further competition experiments indicate that ArlP recognizes neutral and aromatic amino acids with an unbranched β-carbon atom.
|Pages (from-to)||73 - 80|
|Number of pages||8|
|Journal||Biochimica et Biophysica Acta-Biomembranes|
|Publication status||Published - 2002|
- Amino acid uptake, Saccharomyces cerevisiae, Gene expression, Penicillium chrysogenum, Amino acid permease
No data available