Publication

Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter

Bystrykh, L. V. & Belderbos, M. E., 5-Apr-2016, Stem Cell Heterogeneity: Methods and Protocols. Turksen, K. (ed.). Springer New York LLC, Vol. 1516. p. 57-89 33 p. (Methods in Molecular Biology; vol. 1516).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Cellular barcoding is a recently rediscovered tool to trace the clonal output of individual cells with genetically distinct and heritable DNA sequences. Each year a few dozens of papers are published using the cellular barcoding technique. Those publications largely focus on mutually related issues, namely: counting cells capable of clonal proliferation and expansion, monitoring clonal dynamics in time, tracing the origin of differentiated cells, characterizing the differentiation potential of stem cells and similar topics. Apart from their biological content, claims and conclusions, these studies show remarkable diversity in technical aspects of the barcoding method and sometimes in major conclusions. Although a diversity of approaches is quite usual in data analysis, deviant handling of barcode data might directly affect experimental results and their biological interpretation. Here, we will describe typical challenges and caveats in cellular barcoding publications available so far.

Original languageEnglish
Title of host publicationStem Cell Heterogeneity
Subtitle of host publicationMethods and Protocols
EditorsKursad Turksen
PublisherSpringer New York LLC
Pages57-89
Number of pages33
Volume1516
ISBN (Electronic)978-1-4939-6550-2
ISBN (Print)978-1-4939-6549-6
Publication statusPublished - 5-Apr-2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1516
ISSN (Print)1064-3745

    Keywords

  • clonal analysis , Hematopoiesis, Stem cells , Barcode Clone, Sequencing, Barcode

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