Publication

CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development

van der Werf, C. S., Hsiao, N-H., Conroy, S., Paredes, J., Ribeiro, A. S., Sribudiani, Y., Seruca, R., Hofstra, R. M. W., Westers, H. & van IJzendoorn, S. C. D., 27-Feb-2013, In : PLoS ONE. 8, 2, 6 p., e54649.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van der Werf, C. S., Hsiao, N-H., Conroy, S., Paredes, J., Ribeiro, A. S., Sribudiani, Y., ... van IJzendoorn, S. C. D. (2013). CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development. PLoS ONE, 8(2), [e54649]. https://doi.org/10.1371/journal.pone.0054649

Author

van der Werf, Christine S. ; Hsiao, Nai-Hua ; Conroy, Siobhan ; Paredes, Joana ; Ribeiro, Ana S. ; Sribudiani, Yunia ; Seruca, Raquel ; Hofstra, Robert M. W. ; Westers, Helga ; van IJzendoorn, Sven C. D. / CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development. In: PLoS ONE. 2013 ; Vol. 8, No. 2.

Harvard

van der Werf, CS, Hsiao, N-H, Conroy, S, Paredes, J, Ribeiro, AS, Sribudiani, Y, Seruca, R, Hofstra, RMW, Westers, H & van IJzendoorn, SCD 2013, 'CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development', PLoS ONE, vol. 8, no. 2, e54649. https://doi.org/10.1371/journal.pone.0054649

Standard

CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development. / van der Werf, Christine S.; Hsiao, Nai-Hua; Conroy, Siobhan; Paredes, Joana; Ribeiro, Ana S.; Sribudiani, Yunia; Seruca, Raquel; Hofstra, Robert M. W.; Westers, Helga; van IJzendoorn, Sven C. D.

In: PLoS ONE, Vol. 8, No. 2, e54649, 27.02.2013.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van der Werf CS, Hsiao N-H, Conroy S, Paredes J, Ribeiro AS, Sribudiani Y et al. CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development. PLoS ONE. 2013 Feb 27;8(2). e54649. https://doi.org/10.1371/journal.pone.0054649


BibTeX

@article{75447a65207b43b695d7ead831bdd092,
title = "CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development",
abstract = "Loss-of-function mutations in CLMP have been found in patients with Congenital Short Bowel Syndrome (CSBS), suggesting that its encoded protein plays a major role in intestinal development. CLMP is a membrane protein that co-localizes with tight junction proteins, but its function is largely unknown. We expressed wild-type (WT)-CLMP and a mutant-CLMP (associated with CSBS) in human intestinal epithelial T84 cells that, as we show here, do not produce endogenous CLMP. We investigated the effects of WT-CLMP and mutant-CLMP proteins on key cellular processes that are important for intestinal epithelial development, including migration, proliferation, viability and transepithelial resistance. Our data showed that expression of WT-CLMP or mutant-CLMP does not affect any of these processes. Moreover, our aggregation assays in CHO cells show that CLMP does not act as a strong adhesion molecule. Thus, our data suggest that, in the in vitro model systems we used, the key processes involved in intestinal epithelial development appear to be unaffected by WT-CLMP or mutant-CLMP. Further research is needed to determine the role of CLMP in the development of the intestine.",
keywords = "TIGHT JUNCTION PROTEIN, ADHERENS JUNCTIONS, FAMILY, EXPRESSION, CELLS",
author = "{van der Werf}, {Christine S.} and Nai-Hua Hsiao and Siobhan Conroy and Joana Paredes and Ribeiro, {Ana S.} and Yunia Sribudiani and Raquel Seruca and Hofstra, {Robert M. W.} and Helga Westers and {van IJzendoorn}, {Sven C. D.}",
year = "2013",
month = "2",
day = "27",
doi = "10.1371/journal.pone.0054649",
language = "English",
volume = "8",
journal = "PLOS-One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "2",

}

RIS

TY - JOUR

T1 - CLMP Is Essential for Intestinal Development, but Does Not Play a Key Role in Cellular Processes Involved in Intestinal Epithelial Development

AU - van der Werf, Christine S.

AU - Hsiao, Nai-Hua

AU - Conroy, Siobhan

AU - Paredes, Joana

AU - Ribeiro, Ana S.

AU - Sribudiani, Yunia

AU - Seruca, Raquel

AU - Hofstra, Robert M. W.

AU - Westers, Helga

AU - van IJzendoorn, Sven C. D.

PY - 2013/2/27

Y1 - 2013/2/27

N2 - Loss-of-function mutations in CLMP have been found in patients with Congenital Short Bowel Syndrome (CSBS), suggesting that its encoded protein plays a major role in intestinal development. CLMP is a membrane protein that co-localizes with tight junction proteins, but its function is largely unknown. We expressed wild-type (WT)-CLMP and a mutant-CLMP (associated with CSBS) in human intestinal epithelial T84 cells that, as we show here, do not produce endogenous CLMP. We investigated the effects of WT-CLMP and mutant-CLMP proteins on key cellular processes that are important for intestinal epithelial development, including migration, proliferation, viability and transepithelial resistance. Our data showed that expression of WT-CLMP or mutant-CLMP does not affect any of these processes. Moreover, our aggregation assays in CHO cells show that CLMP does not act as a strong adhesion molecule. Thus, our data suggest that, in the in vitro model systems we used, the key processes involved in intestinal epithelial development appear to be unaffected by WT-CLMP or mutant-CLMP. Further research is needed to determine the role of CLMP in the development of the intestine.

AB - Loss-of-function mutations in CLMP have been found in patients with Congenital Short Bowel Syndrome (CSBS), suggesting that its encoded protein plays a major role in intestinal development. CLMP is a membrane protein that co-localizes with tight junction proteins, but its function is largely unknown. We expressed wild-type (WT)-CLMP and a mutant-CLMP (associated with CSBS) in human intestinal epithelial T84 cells that, as we show here, do not produce endogenous CLMP. We investigated the effects of WT-CLMP and mutant-CLMP proteins on key cellular processes that are important for intestinal epithelial development, including migration, proliferation, viability and transepithelial resistance. Our data showed that expression of WT-CLMP or mutant-CLMP does not affect any of these processes. Moreover, our aggregation assays in CHO cells show that CLMP does not act as a strong adhesion molecule. Thus, our data suggest that, in the in vitro model systems we used, the key processes involved in intestinal epithelial development appear to be unaffected by WT-CLMP or mutant-CLMP. Further research is needed to determine the role of CLMP in the development of the intestine.

KW - TIGHT JUNCTION PROTEIN

KW - ADHERENS JUNCTIONS

KW - FAMILY

KW - EXPRESSION

KW - CELLS

U2 - 10.1371/journal.pone.0054649

DO - 10.1371/journal.pone.0054649

M3 - Article

VL - 8

JO - PLOS-One

JF - PLOS-One

SN - 1932-6203

IS - 2

M1 - e54649

ER -

ID: 5801741