Cigarette smoke-induced emphysema: A role for the B cell?van der Strate, BWA., Postma, DS., Brandsma, CA., Melgert, BN., Luinge, MA., Geerlings, M., Hylkema, MN., van den Berg, A., Timens, W. & Kerstjens, HAM., 1-Apr-2006, In : American Journal of Respiratory and Critical Care Medicine. 173, 7, p. 751-758 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Rationale: Little is known about what drives the inflammatory reaction in the development of chronic obstructive lung disease. B cells have been found.
Objective: To study the involvement of B cells in the development of emphysema.
Methods: The presence of B-cell follicles and their interaction with other cells were investigated in lungs of patients with chronic obstructive pulmonary disease and of smoking mice. B cells were isolated from lymphoid follicles by laser microdissection and analyzed for the presence of immunoglobulin rearrangements and somatic mutations.
Main Results: Lymphoid follicles consisting of B cells and follicular dendritic cells with adjacent T cells were demonstrated both in the parenchyma and in bronchial walls of patients with emphysema. A clonal process was observed in all follicles and the presence of ongoing somatic mutations was observed in 75% of the follicles, indicating oligoclonal, antigen-specific proliferation. Similar lymphoid follicles were detected in mice that had developed pulmonary inflammation and progressive alveolar airspace enlargement after smoking. The increase in the number of B-cell follicles was progressive with time and correlated with the increase in mean linear intercept. Specific bacterial or viral nucleic acids could not be detected.
Conclusions: B-cell follicles with an oligoclonal, antigen-specific reaction were found in men and mice with emphysema. In mice, the development was progressive with time and correlated with the increase in airspace enlargement. We hypothesize that these B cells contribute to the inflammatory process and/or the development and perpetuation of emphysema by producing antibodies against either tobacco smoke residues or extracellular matrix components.
|Number of pages||8|
|Journal||American Journal of Respiratory and Critical Care Medicine|
|Publication status||Published - 1-Apr-2006|
- autoimmunity, B cell, chronic obstructive pulmonary disease, emphysema, inflammation, OBSTRUCTIVE PULMONARY-DISEASE, MACROPHAGE METALLOELASTASE, RHEUMATOID-ARTHRITIS, AIRWAY INFLAMMATION, EXPOSURE SYSTEMS, LUNG-FUNCTION, COPD, ASSOCIATION, NEUTROPHILS, ACTIVATION