Publication

Cholesterol but not association with detergent resistant membranes is necessary for the transport function of MRP2/ABCC2

Ito, K., Hoekstra, D. & van IJzendoorn, S. C. D., 24-Dec-2008, In : FEBS Letters. 582, 30, p. 4153-4157 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

MRP2(/ABCC2) excretes amphiphilic organic anions into bile, and associates with detergent-resistant bile canalicular membrane domains (DRM). Here, we have evaluated sensitivities of MRP2 transport function and DRM association by titrating the cellular cholesterol content. We demonstrate that the role of cholesterol in the partitioning of MRP2 to DRM can be separated from the role of cholesterol in the function of MRP2, such that (i) cholesterol is not necessary for the polarized distribution of MRP2 at the canalicular membrane, (ii) partitioning into DRM is not required for MRP2 function, yet (iii) the presence of cholesterol is necessary for transport activity. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Original languageEnglish
Pages (from-to)4153-4157
Number of pages5
JournalFEBS Letters
Volume582
Issue number30
Publication statusPublished - 24-Dec-2008

    Keywords

  • ABCC2, HepG2, Raft, Triton X-100 insoluble fraction, Detergent resistant membrane, TAUROURSODEOXYCHOLIC ACID INSERTS, CHOLESTATIC RAT-LIVER, P-GLYCOPROTEIN, CANALICULAR MEMBRANES, LIPID MICRODOMAINS, DRUG-RESISTANCE, EXPORT PUMP, MRP2, PROTEINS, CELLS

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