Publication

Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi

Ul Haq, I., da Rocha Calixto, R. O., Yang, P., Pires dos Santos, G. M., Barreto-Bergter, E., van Elsas, J. D. & de Boer, W., Nov-2016, In : FEMS Microbial Ecology. 92, 11, 14 p., 164.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ul Haq, I., da Rocha Calixto, R. O., Yang, P., Pires dos Santos, G. M., Barreto-Bergter, E., van Elsas, J. D., & de Boer, W. (2016). Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi. FEMS Microbial Ecology, 92(11), [164]. https://doi.org/10.1093/femsec/fiw164

Author

Ul Haq, Irshad ; da Rocha Calixto, Renata Oliveira ; Yang, Pu ; Pires dos Santos, Giulia Maria ; Barreto-Bergter, Eliana ; van Elsas, Jan Dirk ; de Boer, Wietse. / Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi. In: FEMS Microbial Ecology. 2016 ; Vol. 92, No. 11.

Harvard

Ul Haq, I, da Rocha Calixto, RO, Yang, P, Pires dos Santos, GM, Barreto-Bergter, E, van Elsas, JD & de Boer, W 2016, 'Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi', FEMS Microbial Ecology, vol. 92, no. 11, 164. https://doi.org/10.1093/femsec/fiw164

Standard

Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi. / Ul Haq, Irshad; da Rocha Calixto, Renata Oliveira; Yang, Pu; Pires dos Santos, Giulia Maria; Barreto-Bergter, Eliana; van Elsas, Jan Dirk; de Boer, Wietse.

In: FEMS Microbial Ecology, Vol. 92, No. 11, 164, 11.2016.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ul Haq I, da Rocha Calixto RO, Yang P, Pires dos Santos GM, Barreto-Bergter E, van Elsas JD et al. Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi. FEMS Microbial Ecology. 2016 Nov;92(11). 164. https://doi.org/10.1093/femsec/fiw164


BibTeX

@article{56a7454aa39d4d49b55e4bc09c8b555c,
title = "Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi",
abstract = "Burkholderia terrae BS001 has previously been proposed to be a 'generalist' associate of soil fungi, but its strategies of interaction have been largely ignored. Here, we studied the chemotactic behavior of B. terrae BS001 towards Lyophyllum sp. strain Karsten and Trichoderma asperellum 302 and the role of fungal surface molecules in their physical interaction with the bacteria. To assess the involvement of the type 3 secretion system (T3SS), wild-type strain BS001 and T3SS mutant strain BS001-Delta sctD were used in the experiments. First, the two fungi showed divergent behavior when confronted with B. terrae BS001 on soil extract agar medium. Lyophyllum sp. strain Karsten revealed slow growth towards the bacterium, whereas T. asperellum 302 grew avidly over it. Both on soil extract and M9 agar, B. terrae BS001 and BS001-Delta sctD moved chemotactically towards the hyphae of both fungi, with a stronger response to Lyophyllum sp. strain Karsten than to T. asperellum 302. The presence of a progressively increasing glycerol level in the M9 agar enhanced the level of movement. Different oxalic acid concentrations exerted varied effects, with a significantly raised chemotactic response at lower, and a subdued response at higher concentrations. Testing of the adherence of B. terrae BS001 and BS001-Delta sctD to Lyophyllum sp. strain Karsten and to cell envelope-extracted ceramide monohexosides (CMHs) revealed that CMHs in both conidia and hyphae could bind strain BS001 cells. As BS001-Delta sctD adhered significantly less to the CMHs than BS001, the T3SS was presumed to have a role in the interaction. In contrast, such avid adherence was not detected with T. asperellum 302. Thus, B. terrae BS001 shows a behavior characterized by swimming towards Lyophyllum sp. strain Karsten and T. asperellum 302 and attachment to the CMH moiety in the cell envelope, in particular of the former.",
keywords = "chemotaxis, bacterial-fungal interaction, Lyophyllum sp, strain Karsten, Trichoderma asperellum 302, ceramide monohexosides, SP STRAIN KARSTEN, CELL-WALL, MONOCLONAL-ANTIBODY, ASPERGILLUS-FUMIGATUS, STRUCTURAL-ANALYSIS, GROWTH, BACTERIA, GLUCOSYLCERAMIDES, ACID, GLYCOSPHINGOLIPIDS",
author = "{Ul Haq}, Irshad and {da Rocha Calixto}, {Renata Oliveira} and Pu Yang and {Pires dos Santos}, {Giulia Maria} and Eliana Barreto-Bergter and {van Elsas}, {Jan Dirk} and {de Boer}, Wietse",
year = "2016",
month = "11",
doi = "10.1093/femsec/fiw164",
language = "English",
volume = "92",
journal = "FEMS Microbial Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "11",

}

RIS

TY - JOUR

T1 - Chemotaxis and adherence to fungal surfaces are key components of the behavioral response of Burkholderia terrae BS001 to two selected soil fungi

AU - Ul Haq, Irshad

AU - da Rocha Calixto, Renata Oliveira

AU - Yang, Pu

AU - Pires dos Santos, Giulia Maria

AU - Barreto-Bergter, Eliana

AU - van Elsas, Jan Dirk

AU - de Boer, Wietse

PY - 2016/11

Y1 - 2016/11

N2 - Burkholderia terrae BS001 has previously been proposed to be a 'generalist' associate of soil fungi, but its strategies of interaction have been largely ignored. Here, we studied the chemotactic behavior of B. terrae BS001 towards Lyophyllum sp. strain Karsten and Trichoderma asperellum 302 and the role of fungal surface molecules in their physical interaction with the bacteria. To assess the involvement of the type 3 secretion system (T3SS), wild-type strain BS001 and T3SS mutant strain BS001-Delta sctD were used in the experiments. First, the two fungi showed divergent behavior when confronted with B. terrae BS001 on soil extract agar medium. Lyophyllum sp. strain Karsten revealed slow growth towards the bacterium, whereas T. asperellum 302 grew avidly over it. Both on soil extract and M9 agar, B. terrae BS001 and BS001-Delta sctD moved chemotactically towards the hyphae of both fungi, with a stronger response to Lyophyllum sp. strain Karsten than to T. asperellum 302. The presence of a progressively increasing glycerol level in the M9 agar enhanced the level of movement. Different oxalic acid concentrations exerted varied effects, with a significantly raised chemotactic response at lower, and a subdued response at higher concentrations. Testing of the adherence of B. terrae BS001 and BS001-Delta sctD to Lyophyllum sp. strain Karsten and to cell envelope-extracted ceramide monohexosides (CMHs) revealed that CMHs in both conidia and hyphae could bind strain BS001 cells. As BS001-Delta sctD adhered significantly less to the CMHs than BS001, the T3SS was presumed to have a role in the interaction. In contrast, such avid adherence was not detected with T. asperellum 302. Thus, B. terrae BS001 shows a behavior characterized by swimming towards Lyophyllum sp. strain Karsten and T. asperellum 302 and attachment to the CMH moiety in the cell envelope, in particular of the former.

AB - Burkholderia terrae BS001 has previously been proposed to be a 'generalist' associate of soil fungi, but its strategies of interaction have been largely ignored. Here, we studied the chemotactic behavior of B. terrae BS001 towards Lyophyllum sp. strain Karsten and Trichoderma asperellum 302 and the role of fungal surface molecules in their physical interaction with the bacteria. To assess the involvement of the type 3 secretion system (T3SS), wild-type strain BS001 and T3SS mutant strain BS001-Delta sctD were used in the experiments. First, the two fungi showed divergent behavior when confronted with B. terrae BS001 on soil extract agar medium. Lyophyllum sp. strain Karsten revealed slow growth towards the bacterium, whereas T. asperellum 302 grew avidly over it. Both on soil extract and M9 agar, B. terrae BS001 and BS001-Delta sctD moved chemotactically towards the hyphae of both fungi, with a stronger response to Lyophyllum sp. strain Karsten than to T. asperellum 302. The presence of a progressively increasing glycerol level in the M9 agar enhanced the level of movement. Different oxalic acid concentrations exerted varied effects, with a significantly raised chemotactic response at lower, and a subdued response at higher concentrations. Testing of the adherence of B. terrae BS001 and BS001-Delta sctD to Lyophyllum sp. strain Karsten and to cell envelope-extracted ceramide monohexosides (CMHs) revealed that CMHs in both conidia and hyphae could bind strain BS001 cells. As BS001-Delta sctD adhered significantly less to the CMHs than BS001, the T3SS was presumed to have a role in the interaction. In contrast, such avid adherence was not detected with T. asperellum 302. Thus, B. terrae BS001 shows a behavior characterized by swimming towards Lyophyllum sp. strain Karsten and T. asperellum 302 and attachment to the CMH moiety in the cell envelope, in particular of the former.

KW - chemotaxis

KW - bacterial-fungal interaction

KW - Lyophyllum sp

KW - strain Karsten

KW - Trichoderma asperellum 302

KW - ceramide monohexosides

KW - SP STRAIN KARSTEN

KW - CELL-WALL

KW - MONOCLONAL-ANTIBODY

KW - ASPERGILLUS-FUMIGATUS

KW - STRUCTURAL-ANALYSIS

KW - GROWTH

KW - BACTERIA

KW - GLUCOSYLCERAMIDES

KW - ACID

KW - GLYCOSPHINGOLIPIDS

U2 - 10.1093/femsec/fiw164

DO - 10.1093/femsec/fiw164

M3 - Article

VL - 92

JO - FEMS Microbial Ecology

JF - FEMS Microbial Ecology

SN - 0168-6496

IS - 11

M1 - 164

ER -

ID: 37853856