Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signalingKeizer-Gunnink, I., Kortholt, A. & Van Haastert, P. J. M., 21-May-2007, In : Journal of Cell Biology. 177, 4, p. 579-585 7 p.
Research output: Contribution to journal › Article › Academic › peer-review
During embryonic development, cell movement is orchestrated by a multitude of attractants and repellents. Chemoattractants applied as a gradient, such as cAMP with Dictyostelium discoideum or fMLP with neutrophils, induce the activation of phospholipase C ( PLC) and phosphoinositide 3 (PI3)-kinase at the front of the cell, leading to the localized depletion of phosphatidylinositol 4,5- bisphosphate (PI[4,5]P-2) and the accumulation of phosphatidylinositol- 3,4,5-trisphosphate (PI[3,4,5]P-3). Using D. discoideum, we show that chemorepellent cAMP analogues induce localized inhibition of PLC, thereby reversing the polarity of PI( 4,5)P-2. This leads to the accumulation of PI( 3,4,5) P3 at the rear of the cell, and chemotaxis occurs away from the source. We conclude that a PLC polarity switch controls the response to attractants and repellents.
|Number of pages||7|
|Journal||Journal of Cell Biology|
|Publication status||Published - 21-May-2007|
- DICTYOSTELIUM-DISCOIDEUM, NEUTROPHIL CHEMOTAXIS, CELL-MOVEMENT, NEGATIVE CHEMOTAXIS, DISTINCT ROLES, LEADING-EDGE, PROTEIN, ANTAGONISTS, INHIBITION, EXPRESSION