Publication

Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution

Duclos, G. E., Teixeira, V. H., Autissier, P., Gesthalter, Y. B., Reinders-Luinge, M. A., Terrano, R., Dumas, Y. M., Liu, G., Mazzilli, S. A., Brandsma, C-A., van den Berge, M., Janes, S. M., Timens, W., Lenburg, M. E., Spira, A., Campbell, J. D. & Beane, J., Dec-2019, In : Science Advances. 5, 12, 11 p., 3413.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Duclos, G. E., Teixeira, V. H., Autissier, P., Gesthalter, Y. B., Reinders-Luinge, M. A., Terrano, R., ... Beane, J. (2019). Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution. Science Advances, 5(12), [3413]. https://doi.org/10.1126/sciadv.aaw3413

Author

Duclos, Grant E ; Teixeira, Vitor H ; Autissier, Patrick ; Gesthalter, Yaron B ; Reinders-Luinge, Marjan A ; Terrano, Robert ; Dumas, Yves M ; Liu, Gang ; Mazzilli, Sarah A ; Brandsma, Corry-Anke ; van den Berge, Maarten ; Janes, Sam M ; Timens, Wim ; Lenburg, Marc E ; Spira, Avrum ; Campbell, Joshua D ; Beane, Jennifer. / Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution. In: Science Advances. 2019 ; Vol. 5, No. 12.

Harvard

Duclos, GE, Teixeira, VH, Autissier, P, Gesthalter, YB, Reinders-Luinge, MA, Terrano, R, Dumas, YM, Liu, G, Mazzilli, SA, Brandsma, C-A, van den Berge, M, Janes, SM, Timens, W, Lenburg, ME, Spira, A, Campbell, JD & Beane, J 2019, 'Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution', Science Advances, vol. 5, no. 12, 3413. https://doi.org/10.1126/sciadv.aaw3413

Standard

Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution. / Duclos, Grant E; Teixeira, Vitor H; Autissier, Patrick; Gesthalter, Yaron B; Reinders-Luinge, Marjan A; Terrano, Robert; Dumas, Yves M; Liu, Gang; Mazzilli, Sarah A; Brandsma, Corry-Anke; van den Berge, Maarten; Janes, Sam M; Timens, Wim; Lenburg, Marc E; Spira, Avrum; Campbell, Joshua D; Beane, Jennifer.

In: Science Advances, Vol. 5, No. 12, 3413, 12.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Duclos GE, Teixeira VH, Autissier P, Gesthalter YB, Reinders-Luinge MA, Terrano R et al. Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution. Science Advances. 2019 Dec;5(12). 3413. https://doi.org/10.1126/sciadv.aaw3413


BibTeX

@article{97422db5df6d4c328357b7c1ac34c0e3,
title = "Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution",
abstract = "The human bronchial epithelium is composed of multiple distinct cell types that cooperate to defend against environmental insults. While studies have shown that smoking alters bronchial epithelial function and morphology, its precise effects on specific cell types and overall tissue composition are unclear. We used single-cell RNA sequencing to profile bronchial epithelial cells from six never and six current smokers. Unsupervised analyses led to the characterization of a set of toxin metabolism genes that localized to smoker ciliated cells, tissue remodeling associated with a loss of club cells and extensive goblet cell hyperplasia, and a previously unidentified peri-goblet epithelial subpopulation in smokers who expressed a marker of bronchial premalignant lesions. Our data demonstrate that smoke exposure drives a complex landscape of cellular alterations that may prime the human bronchial epithelium for disease.",
author = "Duclos, {Grant E} and Teixeira, {Vitor H} and Patrick Autissier and Gesthalter, {Yaron B} and Reinders-Luinge, {Marjan A} and Robert Terrano and Dumas, {Yves M} and Gang Liu and Mazzilli, {Sarah A} and Corry-Anke Brandsma and {van den Berge}, Maarten and Janes, {Sam M} and Wim Timens and Lenburg, {Marc E} and Avrum Spira and Campbell, {Joshua D} and Jennifer Beane",
note = "Copyright {\circledC} 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).",
year = "2019",
month = "12",
doi = "10.1126/sciadv.aaw3413",
language = "English",
volume = "5",
journal = "Science Advances",
issn = "2375-2548",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "12",

}

RIS

TY - JOUR

T1 - Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution

AU - Duclos, Grant E

AU - Teixeira, Vitor H

AU - Autissier, Patrick

AU - Gesthalter, Yaron B

AU - Reinders-Luinge, Marjan A

AU - Terrano, Robert

AU - Dumas, Yves M

AU - Liu, Gang

AU - Mazzilli, Sarah A

AU - Brandsma, Corry-Anke

AU - van den Berge, Maarten

AU - Janes, Sam M

AU - Timens, Wim

AU - Lenburg, Marc E

AU - Spira, Avrum

AU - Campbell, Joshua D

AU - Beane, Jennifer

N1 - Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

PY - 2019/12

Y1 - 2019/12

N2 - The human bronchial epithelium is composed of multiple distinct cell types that cooperate to defend against environmental insults. While studies have shown that smoking alters bronchial epithelial function and morphology, its precise effects on specific cell types and overall tissue composition are unclear. We used single-cell RNA sequencing to profile bronchial epithelial cells from six never and six current smokers. Unsupervised analyses led to the characterization of a set of toxin metabolism genes that localized to smoker ciliated cells, tissue remodeling associated with a loss of club cells and extensive goblet cell hyperplasia, and a previously unidentified peri-goblet epithelial subpopulation in smokers who expressed a marker of bronchial premalignant lesions. Our data demonstrate that smoke exposure drives a complex landscape of cellular alterations that may prime the human bronchial epithelium for disease.

AB - The human bronchial epithelium is composed of multiple distinct cell types that cooperate to defend against environmental insults. While studies have shown that smoking alters bronchial epithelial function and morphology, its precise effects on specific cell types and overall tissue composition are unclear. We used single-cell RNA sequencing to profile bronchial epithelial cells from six never and six current smokers. Unsupervised analyses led to the characterization of a set of toxin metabolism genes that localized to smoker ciliated cells, tissue remodeling associated with a loss of club cells and extensive goblet cell hyperplasia, and a previously unidentified peri-goblet epithelial subpopulation in smokers who expressed a marker of bronchial premalignant lesions. Our data demonstrate that smoke exposure drives a complex landscape of cellular alterations that may prime the human bronchial epithelium for disease.

U2 - 10.1126/sciadv.aaw3413

DO - 10.1126/sciadv.aaw3413

M3 - Article

C2 - 31844660

VL - 5

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 12

M1 - 3413

ER -

ID: 109870590