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Characterization of the supporting role of SecE in protein translocation

Lycklama a Nijeholt, J. A., de Keyzer, J., Prabudiansyah, I. & Driessen, A. J. M., 17-Sep-2013, In : FEBS Letters. 587, 18, p. 3083-3088 6 p.

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  • Characterization of the supporting role of SecE in protein translocation

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DOI

SecYEG functions as a membrane channel for protein export. SecY constitutes the protein-conducting pore, which is enwrapped by SecE in a V-shaped manner. In its minimal form SecE consists of a single transmembrane segment that is connected to a surface-exposed amphipathic a-helix via a flexible hinge. These two domains are the major sites of interaction between SecE and SecY. Specific cleavage of SecE at the hinge region, which destroys the interaction between the two SecE domains, reduced translocation. When SecE and SecY were disulfide bonded at the two sites of interaction, protein translocation was not affected. This suggests that the SecY and SecE interactions are static, while the hinge region provides flexibility to allow the SecY pore to open. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Original languageEnglish
Pages (from-to)3083-3088
Number of pages6
JournalFEBS Letters
Volume587
Issue number18
Publication statusPublished - 17-Sep-2013

    Keywords

  • Protein translocation, Membrane transport, SecY, SecE, SecA, CONSERVED CYTOPLASMIC REGION, ESCHERICHIA-COLI, PREPROTEIN TRANSLOCASE, MEMBRANE COMPONENT, CROSS-LINKING, MACHINERY

ID: 5950847