Characterization of the Roco Protein Family in Dictyostelium discoideumvan Egmond, W. N. & van Haastert, P. J. M., May-2010, In : Eukaryotic Cell. 9, 5, p. 751-761 11 p.
Research output: Contribution to journal › Article › Academic › peer-review
The Roco family consists of multidomain Ras-GTPases that include LRRK2, a protein mutated in familial Parkinson's disease. The genome of the cellular slime mold Dictyostelium discoideum encodes 11 Roco proteins. To study the functions of these proteins, we systematically knocked out the roco genes. Previously described functions for GbpC, Pats1, and QkgA (Roco1 to Roco3) were confirmed, while novel developmental defects were identified in roco4-and roco11-null cells. Cells lacking Roco11 form larger fruiting bodies than wild-type cells, while roco4-null cells show strong developmental defects during the transition from mound to fruiting body; prestalk cells produce reduced levels of cellulose, leading to unstable stalks that are unable to properly lift the spore head. Detailed phylogenetic analysis of four slime mold species reveals that QkgA and Roco11 evolved relatively late by duplication of an ancestor roco4 gene (later than similar to 300 million years ago), contrary to the situation with other roco genes, which were already present before the split of the common ancestor of D. discoideum and Polysphondylium pallidum (before similar to 600 million years ago). Together, our data show that the Dictyostelium Roco proteins serve a surprisingly diverse set of functions and highlight Roco4 as a key protein for proper stalk cell formation.
|Number of pages||11|
|Publication status||Published - May-2010|
- DISEASE-ASSOCIATED MUTATIONS, PARKINSONS-DISEASE, GENE LRRK2, KINASE, IDENTIFICATION, CHEMOTAXIS, INSIGHTS, PATHWAY, DOMAIN, DEATH