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Characterization of the in vitro, ex vivo, and in vivo Efficacy of the Antimicrobial Peptide DPK-060 Used for Topical Treatment

Hakansson, J., Ringstad, L., Umerska, A., Johansson, J., Andersson, T., Boge, L., Rozenbaum, R. T., Sharma, P. K., Tollback, P., Bjorn, C., Saulnier, P. & Mahlapuu, M., 28-May-2019, In : Frontiers in Cellular and Infection Microbiology. 9, 11 p., 174.

Research output: Contribution to journalArticleAcademicpeer-review

  • Joakim Hakansson
  • Lovisa Ringstad
  • Anita Umerska
  • Jenny Johansson
  • Therese Andersson
  • Lukas Boge
  • Rene T. Rozenbaum
  • Prashant K. Sharma
  • Petter Tollback
  • Camilla Bjorn
  • Patrick Saulnier
  • Margit Mahlapuu

Antimicrobial peptides, also known as host defense peptides, have recently emerged as a promising new category of therapeutic agents for the treatment of infectious diseases. This study evaluated the preclinical in vitro, ex vivo, and in vivo antimicrobial activity, as well as the potential to cause skin irritation, of human kininogen-derived antimicrobial peptide DPK-060 in different formulations designed for topical delivery. We found that DPK-060 formulated in acetate buffer or poloxamer gel caused a marked reduction of bacterial counts of Staphylococcus aureus in vitro (minimum microbicidal concentration = 99 or >= 94% reduction in bacterial counts was achieved with 1% DPK-060 at 4 h post-treatment, respectively). Encapsulation of DPK-060 in different types of lipid nanocapsules or cubosomes did not improve the bactericidal potential of the peptide under the applied test conditions. No reduction in cell viability was observed in response to administration of DPK-060 in any of the formulations tested. In conclusion, the present study confirms that DPK-060 has the potential to be an effective and safe drug candidate for the topical treatment of microbial infections; however, adsorption of the peptide to nanocarriers failed to show any additional benefits.

Original languageEnglish
Article number174
Number of pages11
JournalFrontiers in Cellular and Infection Microbiology
Volume9
Publication statusPublished - 28-May-2019

    Keywords

  • antimicrobial peptides, DPK-060, skin infections, lipid nanocapsules, cubosomes, LIPID NANOCAPSULES, SYNERGISTIC INTERACTIONS, HUMAN LACTOFERRIN, SKIN PENETRATION, INFECTIONS, PLECTASIN, MONOOLEIN, CUBOSOMES, DELIVERY, MODEL

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