Publication

Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions

Krooneman, J., Sliekers, AO., Gomes, TMP., Forney, LJ. & Gottschal, JC., Apr-2000, In : FEMS Microbial Ecology. 32, 1, p. 53-59 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Krooneman, J., Sliekers, AO., Gomes, TMP., Forney, LJ., & Gottschal, JC. (2000). Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions. FEMS Microbial Ecology, 32(1), 53-59.

Author

Krooneman, J ; Sliekers, AO ; Gomes, TMP ; Forney, LJ ; Gottschal, JC. / Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions. In: FEMS Microbial Ecology. 2000 ; Vol. 32, No. 1. pp. 53-59.

Harvard

Krooneman, J, Sliekers, AO, Gomes, TMP, Forney, LJ & Gottschal, JC 2000, 'Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions', FEMS Microbial Ecology, vol. 32, no. 1, pp. 53-59.

Standard

Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions. / Krooneman, J; Sliekers, AO; Gomes, TMP; Forney, LJ; Gottschal, JC.

In: FEMS Microbial Ecology, Vol. 32, No. 1, 04.2000, p. 53-59.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Krooneman J, Sliekers AO, Gomes TMP, Forney LJ, Gottschal JC. Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions. FEMS Microbial Ecology. 2000 Apr;32(1):53-59.


BibTeX

@article{4b42f59d4e9849c49860cfb7b8ea9dea,
title = "Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions",
abstract = "The rates of bacterial growth in nature are often restricted by low concentrations of oxygen or carbon substrates. In the present study the metabolic properties of 24 isolates that had been isolated using various concentrations of 3-chlorobenzoate, benzoate and oxygen as well as using continuous culture at high and low growth rates were determined to investigate the effects of these parameters on the metabolism of monoaromatic compounds. Bacteria were enriched from different sampling sites and subsequently isolated. In batch culture this was done both under low oxygen (2{\%} O-2) and air-saturated concentrations. Chemostat enrichments were performed under either oxygen or 3-chlorobenzoate limiting conditions. Bacteria metabolizing aromatics with gentisate or protocatechuate as intermediates (gp bacteria) as well as bacteria metabolizing aromatic compounds via catechols (cat bacteria) were isolated from batch cultures when either benzoate or 3CBA were used as C sources, regardless of the enrichment conditions applied. In contrast, enrichments performed in chemostats at low dilution rates resulted in gp-type organisms only, whereas at high dilution rates cat-type organisms were enriched, irrespective of the oxygen and 3-chlorobenzoate concentration used during enrichment. It is noteworthy that the gp-type of bacteria possessed relatively low Irm, values on 3CBA and benzoate along with relatively high substrate and oxygen affinities for these compounds. This is in contrast with cat-type of bacteria, which seemed to be characterized by high maximum specific growth rates on the aromatic substrates and relatively high apparent half saturation constants. In contrast, bacteria degrading chlorobenzoate via gentisate or protocatechuate may possibly be better adapted to conditions leading to growth at reduced rates such as low oxygen and low substrate concentrations. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
keywords = "3-chlorobenzoate, substrate limitation, hypoxic condition, catechol pathway, gentisate, protocatechuate pathway, OXYGEN CONCENTRATIONS, MICROBIAL-GROWTH, DEGRADATION, CATECHOL, KINETICS",
author = "J Krooneman and AO Sliekers and TMP Gomes and LJ Forney and JC Gottschal",
year = "2000",
month = "4",
language = "English",
volume = "32",
pages = "53--59",
journal = "FEMS Microbial Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Characterization of 3-chlorobenzoate degrading aerobic bacteria isolated under various environmental conditions

AU - Krooneman, J

AU - Sliekers, AO

AU - Gomes, TMP

AU - Forney, LJ

AU - Gottschal, JC

PY - 2000/4

Y1 - 2000/4

N2 - The rates of bacterial growth in nature are often restricted by low concentrations of oxygen or carbon substrates. In the present study the metabolic properties of 24 isolates that had been isolated using various concentrations of 3-chlorobenzoate, benzoate and oxygen as well as using continuous culture at high and low growth rates were determined to investigate the effects of these parameters on the metabolism of monoaromatic compounds. Bacteria were enriched from different sampling sites and subsequently isolated. In batch culture this was done both under low oxygen (2% O-2) and air-saturated concentrations. Chemostat enrichments were performed under either oxygen or 3-chlorobenzoate limiting conditions. Bacteria metabolizing aromatics with gentisate or protocatechuate as intermediates (gp bacteria) as well as bacteria metabolizing aromatic compounds via catechols (cat bacteria) were isolated from batch cultures when either benzoate or 3CBA were used as C sources, regardless of the enrichment conditions applied. In contrast, enrichments performed in chemostats at low dilution rates resulted in gp-type organisms only, whereas at high dilution rates cat-type organisms were enriched, irrespective of the oxygen and 3-chlorobenzoate concentration used during enrichment. It is noteworthy that the gp-type of bacteria possessed relatively low Irm, values on 3CBA and benzoate along with relatively high substrate and oxygen affinities for these compounds. This is in contrast with cat-type of bacteria, which seemed to be characterized by high maximum specific growth rates on the aromatic substrates and relatively high apparent half saturation constants. In contrast, bacteria degrading chlorobenzoate via gentisate or protocatechuate may possibly be better adapted to conditions leading to growth at reduced rates such as low oxygen and low substrate concentrations. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The rates of bacterial growth in nature are often restricted by low concentrations of oxygen or carbon substrates. In the present study the metabolic properties of 24 isolates that had been isolated using various concentrations of 3-chlorobenzoate, benzoate and oxygen as well as using continuous culture at high and low growth rates were determined to investigate the effects of these parameters on the metabolism of monoaromatic compounds. Bacteria were enriched from different sampling sites and subsequently isolated. In batch culture this was done both under low oxygen (2% O-2) and air-saturated concentrations. Chemostat enrichments were performed under either oxygen or 3-chlorobenzoate limiting conditions. Bacteria metabolizing aromatics with gentisate or protocatechuate as intermediates (gp bacteria) as well as bacteria metabolizing aromatic compounds via catechols (cat bacteria) were isolated from batch cultures when either benzoate or 3CBA were used as C sources, regardless of the enrichment conditions applied. In contrast, enrichments performed in chemostats at low dilution rates resulted in gp-type organisms only, whereas at high dilution rates cat-type organisms were enriched, irrespective of the oxygen and 3-chlorobenzoate concentration used during enrichment. It is noteworthy that the gp-type of bacteria possessed relatively low Irm, values on 3CBA and benzoate along with relatively high substrate and oxygen affinities for these compounds. This is in contrast with cat-type of bacteria, which seemed to be characterized by high maximum specific growth rates on the aromatic substrates and relatively high apparent half saturation constants. In contrast, bacteria degrading chlorobenzoate via gentisate or protocatechuate may possibly be better adapted to conditions leading to growth at reduced rates such as low oxygen and low substrate concentrations. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - 3-chlorobenzoate

KW - substrate limitation

KW - hypoxic condition

KW - catechol pathway

KW - gentisate

KW - protocatechuate pathway

KW - OXYGEN CONCENTRATIONS

KW - MICROBIAL-GROWTH

KW - DEGRADATION

KW - CATECHOL

KW - KINETICS

M3 - Article

VL - 32

SP - 53

EP - 59

JO - FEMS Microbial Ecology

JF - FEMS Microbial Ecology

SN - 0168-6496

IS - 1

ER -

ID: 3860183