Publication

Changes in oral microflora after full-mouth tooth extraction: A prospective cohort study

de Waal, Y. C. M., Winkel, E. G., Raangs, G. C., van der Vusse, M. L., Rossen, J. W. A. & van Winkelhoff, A. J., Oct-2014, In : Journal of Clinical Periodontology. 41, 10, p. 981-989 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

de Waal, Y. C. M., Winkel, E. G., Raangs, G. C., van der Vusse, M. L., Rossen, J. W. A., & van Winkelhoff, A. J. (2014). Changes in oral microflora after full-mouth tooth extraction: A prospective cohort study. Journal of Clinical Periodontology, 41(10), 981-989. https://doi.org/10.1111/jcpe.12297

Author

de Waal, Yvonne C M ; Winkel, Edwin G ; Raangs, Gerwin C ; van der Vusse, Marleen L ; Rossen, John W A ; van Winkelhoff, Arie Jan. / Changes in oral microflora after full-mouth tooth extraction : A prospective cohort study. In: Journal of Clinical Periodontology. 2014 ; Vol. 41, No. 10. pp. 981-989.

Harvard

de Waal, YCM, Winkel, EG, Raangs, GC, van der Vusse, ML, Rossen, JWA & van Winkelhoff, AJ 2014, 'Changes in oral microflora after full-mouth tooth extraction: A prospective cohort study', Journal of Clinical Periodontology, vol. 41, no. 10, pp. 981-989. https://doi.org/10.1111/jcpe.12297

Standard

Changes in oral microflora after full-mouth tooth extraction : A prospective cohort study. / de Waal, Yvonne C M; Winkel, Edwin G; Raangs, Gerwin C; van der Vusse, Marleen L; Rossen, John W A; van Winkelhoff, Arie Jan.

In: Journal of Clinical Periodontology, Vol. 41, No. 10, 10.2014, p. 981-989.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

de Waal YCM, Winkel EG, Raangs GC, van der Vusse ML, Rossen JWA, van Winkelhoff AJ. Changes in oral microflora after full-mouth tooth extraction: A prospective cohort study. Journal of Clinical Periodontology. 2014 Oct;41(10):981-989. https://doi.org/10.1111/jcpe.12297


BibTeX

@article{7d36d5258bf84a2692fe871ae051c9de,
title = "Changes in oral microflora after full-mouth tooth extraction: A prospective cohort study",
abstract = "AIM: The aim of the study was to evaluate the effect of full-mouth tooth extraction on the oral microflora, with emphasis on the presence and load of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.MATERIAL AND METHODS: Adult patients (n = 30), with moderate to advanced periodontitis and scheduled for full-mouth tooth extraction, were consecutively selected. Prior to and 1 and 3 months after full-mouth tooth extraction saliva, tongue, buccal and gingival mucosa and subgingival plaque/prosthesis samples were obtained. Aerobic and anaerobic culture techniques and quantitative real-time polymerase chain reaction (qPCR) were employed for the detection of oral pathogens.RESULTS: Full-mouth tooth extraction resulted in reduction below detection level of A. actinomycetemcomitans and P. gingivalis in 15 of 16 and 8 of 16 previously positive patients using culture techniques and qPCR, respectively. Those patients remaining qPCR positive showed a significant reduction in load of these bacteria.CONCLUSION: Full-mouth tooth extraction significantly changes the oral microflora. These changes include reduction of A. actinomycetemcomitans and P. gingivalis, frequently to levels below detection threshold. In some patients, A. actinomycetemcomitans and P. gingivalis can persist in the edentulous oral cavity up to 3 months after full-mouth tooth extraction.",
keywords = "edentulous mouth, microbiology, periodontal diseases, tooth extraction, REAL-TIME PCR, PUTATIVE PERIODONTAL PATHOGENS, POLYMERASE-CHAIN-REACTION, MUCOUS-MEMBRANES, ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, EDENTULOUS PATIENTS, PORPHYROMONAS-GINGIVALIS, SUBGINGIVAL MICROBIOTA, CHILDREN, IMPLANTS",
author = "{de Waal}, {Yvonne C M} and Winkel, {Edwin G} and Raangs, {Gerwin C} and {van der Vusse}, {Marleen L} and Rossen, {John W A} and {van Winkelhoff}, {Arie Jan}",
note = "{\textcopyright} 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",
year = "2014",
month = oct,
doi = "10.1111/jcpe.12297",
language = "English",
volume = "41",
pages = "981--989",
journal = "Journal of Clinical Periodontology",
issn = "0303-6979",
publisher = "Wiley",
number = "10",

}

RIS

TY - JOUR

T1 - Changes in oral microflora after full-mouth tooth extraction

T2 - A prospective cohort study

AU - de Waal, Yvonne C M

AU - Winkel, Edwin G

AU - Raangs, Gerwin C

AU - van der Vusse, Marleen L

AU - Rossen, John W A

AU - van Winkelhoff, Arie Jan

N1 - © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PY - 2014/10

Y1 - 2014/10

N2 - AIM: The aim of the study was to evaluate the effect of full-mouth tooth extraction on the oral microflora, with emphasis on the presence and load of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.MATERIAL AND METHODS: Adult patients (n = 30), with moderate to advanced periodontitis and scheduled for full-mouth tooth extraction, were consecutively selected. Prior to and 1 and 3 months after full-mouth tooth extraction saliva, tongue, buccal and gingival mucosa and subgingival plaque/prosthesis samples were obtained. Aerobic and anaerobic culture techniques and quantitative real-time polymerase chain reaction (qPCR) were employed for the detection of oral pathogens.RESULTS: Full-mouth tooth extraction resulted in reduction below detection level of A. actinomycetemcomitans and P. gingivalis in 15 of 16 and 8 of 16 previously positive patients using culture techniques and qPCR, respectively. Those patients remaining qPCR positive showed a significant reduction in load of these bacteria.CONCLUSION: Full-mouth tooth extraction significantly changes the oral microflora. These changes include reduction of A. actinomycetemcomitans and P. gingivalis, frequently to levels below detection threshold. In some patients, A. actinomycetemcomitans and P. gingivalis can persist in the edentulous oral cavity up to 3 months after full-mouth tooth extraction.

AB - AIM: The aim of the study was to evaluate the effect of full-mouth tooth extraction on the oral microflora, with emphasis on the presence and load of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.MATERIAL AND METHODS: Adult patients (n = 30), with moderate to advanced periodontitis and scheduled for full-mouth tooth extraction, were consecutively selected. Prior to and 1 and 3 months after full-mouth tooth extraction saliva, tongue, buccal and gingival mucosa and subgingival plaque/prosthesis samples were obtained. Aerobic and anaerobic culture techniques and quantitative real-time polymerase chain reaction (qPCR) were employed for the detection of oral pathogens.RESULTS: Full-mouth tooth extraction resulted in reduction below detection level of A. actinomycetemcomitans and P. gingivalis in 15 of 16 and 8 of 16 previously positive patients using culture techniques and qPCR, respectively. Those patients remaining qPCR positive showed a significant reduction in load of these bacteria.CONCLUSION: Full-mouth tooth extraction significantly changes the oral microflora. These changes include reduction of A. actinomycetemcomitans and P. gingivalis, frequently to levels below detection threshold. In some patients, A. actinomycetemcomitans and P. gingivalis can persist in the edentulous oral cavity up to 3 months after full-mouth tooth extraction.

KW - edentulous mouth

KW - microbiology

KW - periodontal diseases

KW - tooth extraction

KW - REAL-TIME PCR

KW - PUTATIVE PERIODONTAL PATHOGENS

KW - POLYMERASE-CHAIN-REACTION

KW - MUCOUS-MEMBRANES

KW - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

KW - EDENTULOUS PATIENTS

KW - PORPHYROMONAS-GINGIVALIS

KW - SUBGINGIVAL MICROBIOTA

KW - CHILDREN

KW - IMPLANTS

U2 - 10.1111/jcpe.12297

DO - 10.1111/jcpe.12297

M3 - Article

C2 - 25065274

VL - 41

SP - 981

EP - 989

JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

IS - 10

ER -

ID: 13803308