Publication

Cerebral adenosine A1 receptors are upregulated in rodent encephalitis

Paul, S., Khanapur, S., Boersma, W., Sijbesma, J. W., Ishiwata, K., Elsinga, P. H., Meerlo, P., Doorduin, J., Dierckx, R. A. & van Waarde, A., 2014, In : Neuroimage. 92, p. 83-89

Research output: Contribution to journalArticleAcademicpeer-review

APA

Paul, S., Khanapur, S., Boersma, W., Sijbesma, J. W., Ishiwata, K., Elsinga, P. H., ... van Waarde, A. (2014). Cerebral adenosine A1 receptors are upregulated in rodent encephalitis. Neuroimage, 92, 83-89. https://doi.org/10.1016/j.neuroimage.2014.01.054

Author

Paul, Souman ; Khanapur, Shivashankar ; Boersma, Wytske ; Sijbesma, Jurgen W. ; Ishiwata, Kiichi ; Elsinga, Philip H. ; Meerlo, Peter ; Doorduin, Janine ; Dierckx, Rudi A. ; van Waarde, Aren. / Cerebral adenosine A1 receptors are upregulated in rodent encephalitis. In: Neuroimage. 2014 ; Vol. 92. pp. 83-89.

Harvard

Paul, S, Khanapur, S, Boersma, W, Sijbesma, JW, Ishiwata, K, Elsinga, PH, Meerlo, P, Doorduin, J, Dierckx, RA & van Waarde, A 2014, 'Cerebral adenosine A1 receptors are upregulated in rodent encephalitis', Neuroimage, vol. 92, pp. 83-89. https://doi.org/10.1016/j.neuroimage.2014.01.054

Standard

Cerebral adenosine A1 receptors are upregulated in rodent encephalitis. / Paul, Souman; Khanapur, Shivashankar; Boersma, Wytske; Sijbesma, Jurgen W.; Ishiwata, Kiichi; Elsinga, Philip H.; Meerlo, Peter; Doorduin, Janine; Dierckx, Rudi A.; van Waarde, Aren.

In: Neuroimage, Vol. 92, 2014, p. 83-89.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Paul S, Khanapur S, Boersma W, Sijbesma JW, Ishiwata K, Elsinga PH et al. Cerebral adenosine A1 receptors are upregulated in rodent encephalitis. Neuroimage. 2014;92:83-89. https://doi.org/10.1016/j.neuroimage.2014.01.054


BibTeX

@article{254c846b6b1b41a8b2f9ef72f9081588,
title = "Cerebral adenosine A1 receptors are upregulated in rodent encephalitis",
abstract = "Adenosine A(1) receptors (A(1) Rs) are implied in the modulation of neuroinflammation. Activation of cerebral A(1) Rs acts as a brake on the microglial response after traumatic brain injury and has neuroprotective properties in animal models of Parkinson's disease and multiple sclerosis. Neuroinflammatory processes in turn may affect the expression of A1Rs, but the available data is limited and inconsistent. Here, we applied an animal model of encephalitis to assess how neuroinflammation affects the expression of A1 Rs. Two groups of animals were studied: Infected rats (n=7) were intranasally inoculated with herpes simplex virus-1 (HSV-1, 1 x 10(7) plaque forming units), sham-infected rats (n=6) received only phosphate-buffered saline. Six or seven days later, microPET scans (60 min with arterial blood sampling) were made using the tracer 8-dicyclopropyl-1-C-11-methy1-3-propyl-xanthine (C-11-MPDX). Tracer clearance from plasma and partition coefficient (K-1/k(2) estimated from a 2-tissue compartment model fit) were not significantly altered after virus infection. PET tracer distribution volume calculated from a Logan plot was significantly increased in the hippocampus (+37{\%}) and medulla (+27{\%}) of virug infected rats. Tracer binding potential (k(3)/k(4) estimated from the model fit) was significantly increased in the cerebellum (+87{\%}) and the medulla (+148{\%}) which may indicate increased A1R expression. This was confirmed by immunohistochemical analysis showing a strong increase of A1R immunoreactivity in the cerebellum of HSV-1-infected rats. Both the quantitative PET data and immunohistochemical analysis indicate that A1Rs are upregulated in brain areas where active virus is present. (C) 2014 Elsevier Inc. All rights reserved.",
keywords = "encephalitis, herpes simplex virus-1, neuroinflammation, brain, adenosine, adenosine A1 receptors, positron emission tomography, PET, MicroPET",
author = "Souman Paul and Shivashankar Khanapur and Wytske Boersma and Sijbesma, {Jurgen W.} and Kiichi Ishiwata and Elsinga, {Philip H.} and Peter Meerlo and Janine Doorduin and Dierckx, {Rudi A.} and {van Waarde}, Aren",
year = "2014",
doi = "10.1016/j.neuroimage.2014.01.054",
language = "English",
volume = "92",
pages = "83--89",
journal = "Neuroimage",
issn = "1053-8119",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",

}

RIS

TY - JOUR

T1 - Cerebral adenosine A1 receptors are upregulated in rodent encephalitis

AU - Paul, Souman

AU - Khanapur, Shivashankar

AU - Boersma, Wytske

AU - Sijbesma, Jurgen W.

AU - Ishiwata, Kiichi

AU - Elsinga, Philip H.

AU - Meerlo, Peter

AU - Doorduin, Janine

AU - Dierckx, Rudi A.

AU - van Waarde, Aren

PY - 2014

Y1 - 2014

N2 - Adenosine A(1) receptors (A(1) Rs) are implied in the modulation of neuroinflammation. Activation of cerebral A(1) Rs acts as a brake on the microglial response after traumatic brain injury and has neuroprotective properties in animal models of Parkinson's disease and multiple sclerosis. Neuroinflammatory processes in turn may affect the expression of A1Rs, but the available data is limited and inconsistent. Here, we applied an animal model of encephalitis to assess how neuroinflammation affects the expression of A1 Rs. Two groups of animals were studied: Infected rats (n=7) were intranasally inoculated with herpes simplex virus-1 (HSV-1, 1 x 10(7) plaque forming units), sham-infected rats (n=6) received only phosphate-buffered saline. Six or seven days later, microPET scans (60 min with arterial blood sampling) were made using the tracer 8-dicyclopropyl-1-C-11-methy1-3-propyl-xanthine (C-11-MPDX). Tracer clearance from plasma and partition coefficient (K-1/k(2) estimated from a 2-tissue compartment model fit) were not significantly altered after virus infection. PET tracer distribution volume calculated from a Logan plot was significantly increased in the hippocampus (+37%) and medulla (+27%) of virug infected rats. Tracer binding potential (k(3)/k(4) estimated from the model fit) was significantly increased in the cerebellum (+87%) and the medulla (+148%) which may indicate increased A1R expression. This was confirmed by immunohistochemical analysis showing a strong increase of A1R immunoreactivity in the cerebellum of HSV-1-infected rats. Both the quantitative PET data and immunohistochemical analysis indicate that A1Rs are upregulated in brain areas where active virus is present. (C) 2014 Elsevier Inc. All rights reserved.

AB - Adenosine A(1) receptors (A(1) Rs) are implied in the modulation of neuroinflammation. Activation of cerebral A(1) Rs acts as a brake on the microglial response after traumatic brain injury and has neuroprotective properties in animal models of Parkinson's disease and multiple sclerosis. Neuroinflammatory processes in turn may affect the expression of A1Rs, but the available data is limited and inconsistent. Here, we applied an animal model of encephalitis to assess how neuroinflammation affects the expression of A1 Rs. Two groups of animals were studied: Infected rats (n=7) were intranasally inoculated with herpes simplex virus-1 (HSV-1, 1 x 10(7) plaque forming units), sham-infected rats (n=6) received only phosphate-buffered saline. Six or seven days later, microPET scans (60 min with arterial blood sampling) were made using the tracer 8-dicyclopropyl-1-C-11-methy1-3-propyl-xanthine (C-11-MPDX). Tracer clearance from plasma and partition coefficient (K-1/k(2) estimated from a 2-tissue compartment model fit) were not significantly altered after virus infection. PET tracer distribution volume calculated from a Logan plot was significantly increased in the hippocampus (+37%) and medulla (+27%) of virug infected rats. Tracer binding potential (k(3)/k(4) estimated from the model fit) was significantly increased in the cerebellum (+87%) and the medulla (+148%) which may indicate increased A1R expression. This was confirmed by immunohistochemical analysis showing a strong increase of A1R immunoreactivity in the cerebellum of HSV-1-infected rats. Both the quantitative PET data and immunohistochemical analysis indicate that A1Rs are upregulated in brain areas where active virus is present. (C) 2014 Elsevier Inc. All rights reserved.

KW - encephalitis

KW - herpes simplex virus-1

KW - neuroinflammation

KW - brain

KW - adenosine

KW - adenosine A1 receptors

KW - positron emission tomography

KW - PET

KW - MicroPET

U2 - 10.1016/j.neuroimage.2014.01.054

DO - 10.1016/j.neuroimage.2014.01.054

M3 - Article

VL - 92

SP - 83

EP - 89

JO - Neuroimage

JF - Neuroimage

SN - 1053-8119

ER -

ID: 15815142