Publication

Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures

Boender, L. G. M., van Maris, A. J. A., de Hulster, E. A. F., Almering, M. J. H., van der Klei, I. J., Veenhuis, M., de Winde, J. H., Pronk, J. T. & Daran-Lapujade, P., Dec-2011, In : Fems Yeast Research. 11, 8, p. 603-620 18 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

DOI

  • Leonie G. M. Boender
  • Antonius J. A. van Maris
  • Erik A. F. de Hulster
  • Marinka J. H. Almering
  • Ida J. van der Klei
  • Marten Veenhuis
  • Johannes H. de Winde
  • Jack T. Pronk
  • Pascale Daran-Lapujade
Extremely low specific growth rates (below 0.01 h(-1)) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is typically investigated as a result of carbon starvation, cells in retentostat are fed by small, but continuous carbon and energy supply. Yeast cells cultivated near-zero specific growth rates, while metabolically active, exhibited characteristics previously associated with quiescence, including accumulation of storage polymers and an increased expression of genes involved in exit from the cell cycle into G(0). Unexpectedly, analysis of transcriptome data from retentostat and chemostat cultures showed, as specific growth rate was decreased, that quiescence-related transcriptional responses were already set in at specific growth rates above 0.025 h(-1). These observations stress the need for systematic dissection of physiological responses to slow growth, quiescence, ageing and starvation and indicate that controlled cultivation systems such as retentostats can contribute to this goal. Furthermore, cells in retentostat do not (or hardly) divide while remaining metabolically active, which emulates the physiological status of metazoan post-mitotic cells. We propose retentostat as a powerful cultivation tool to investigate chronological ageing-related processes.
Original languageEnglish
Pages (from-to)603-620
Number of pages18
JournalFems Yeast Research
Volume11
Issue number8
Publication statusPublished - Dec-2011

    Keywords

  • retentostat, Saccharomyces cerevisiae, near-zero growth rates, quiescence, chronological ageing, STATIONARY-PHASE, CHEMOSTAT CULTURES, MICROARRAY EXPERIMENTS, NUTRIENT AVAILABILITY, SIGNALING PATHWAYS, GENE-EXPRESSION, STRESS-RESPONSE, PROTEIN-KINASE, AGING RESEARCH, SLOW GROWTH

Download statistics

No data available

ID: 5445887