CD27(+)CD38(hi) B Cell Frequency During Remission Predicts Relapsing Disease in Granulomatosis With Polyangiitis Patientsvon Borstel, A., Land, J., Abdulahad, W. H., Rutgers, A., Stegeman, C. A., Diepstra, A., Heeringa, P. & Sanders, J. S., 24-Sep-2019, In : Frontiers in Immunology. 10, 10 p., 2221.
Research output: Contribution to journal › Article › Academic › peer-review
Background: Granulomatosis with polyangiitis (GPA) patients are prone to disease relapses. We aimed to determine whether GPA patients at risk for relapse can be identified by differences in B cell subset frequencies.
Methods: Eighty-five GPA patients were monitored for a median period of 3.1 years (range: 0.1-6.3). Circulating B cell subset frequencies were analyzed by flow cytometry determining the expression of CD19, CD38, and CD27. B cell subset frequencies at the time of inclusion of future-relapsing (F-R) and non-relapsing (N-R) patients were compared and related to relapse-free survival. Additionally, CD27(+)CD38(hi) B cells were assessed in urine and kidney biopsies from active anti-neutrophil cytoplasmic autoantibody-associated vasculitides (AAV) patients with renal involvement.
Results: Within 1.6 years, 30% of patients experienced a relapse. The CD27(+)CD38(hi) B cell frequency at the time of inclusion was increased in F-R (median: 2.39%) compared to N-R patients (median: 1.03%; p = 0.0025) and a trend was found compared with the HCs (median: 1.33%; p = 0.08). This increased CD27(+)CD38(hi) B cell frequency at inclusion was correlated to decreased relapse-free survival in GPA patients. In addition, 74.7% of patients with an increased CD27(+)CD38(hi) B cell frequency (>= 2.39%) relapsed during follow-up compared to 19.7% of patients with a CD27(+)CD38(hi) B cell frequency of
Conclusions: Our data suggests that having an increased frequency of circulating CD27(+)CD38(hi) B cells during remission is related to a higher relapse risk in GPA patients, and thereforemight be a potentialmarker to identify those GPA patients at risk for relapse.
|Number of pages||10|
|Journal||Frontiers in Immunology|
|Publication status||Published - 24-Sep-2019|
- vasculitis, granulomatosis with polyangiitis, ANCA, B cells, relapse, CYTOPLASMIC AUTOANTIBODIES, WEGENERS-GRANULOMATOSIS, PLASMA-CELLS, VASCULITIS, CYCLOPHOSPHAMIDE, DIFFERENTIATION, SPECIFICITY, ACTIVATION, RITUXIMAB, PROTEIN