Publication

CARD15 gene mutations in periodontitis

Laine, ML., Murillo, LS., Morre, SA., Winkel, EG., Pena, AS. & van Winkelhoff, AJ., Oct-2004, In : Journal of Clinical Periodontology. 31, 10, p. 890-893 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Laine, ML., Murillo, LS., Morre, SA., Winkel, EG., Pena, AS., & van Winkelhoff, AJ. (2004). CARD15 gene mutations in periodontitis. Journal of Clinical Periodontology, 31(10), 890-893. https://doi.org/10.1111/j.1600-051X.2004.00577.x

Author

Laine, ML ; Murillo, LS ; Morre, SA ; Winkel, EG ; Pena, AS ; van Winkelhoff, AJ. / CARD15 gene mutations in periodontitis. In: Journal of Clinical Periodontology. 2004 ; Vol. 31, No. 10. pp. 890-893.

Harvard

Laine, ML, Murillo, LS, Morre, SA, Winkel, EG, Pena, AS & van Winkelhoff, AJ 2004, 'CARD15 gene mutations in periodontitis', Journal of Clinical Periodontology, vol. 31, no. 10, pp. 890-893. https://doi.org/10.1111/j.1600-051X.2004.00577.x

Standard

CARD15 gene mutations in periodontitis. / Laine, ML; Murillo, LS; Morre, SA; Winkel, EG; Pena, AS; van Winkelhoff, AJ.

In: Journal of Clinical Periodontology, Vol. 31, No. 10, 10.2004, p. 890-893.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Laine ML, Murillo LS, Morre SA, Winkel EG, Pena AS, van Winkelhoff AJ. CARD15 gene mutations in periodontitis. Journal of Clinical Periodontology. 2004 Oct;31(10):890-893. https://doi.org/10.1111/j.1600-051X.2004.00577.x


BibTeX

@article{bbac13a131ba4c64a2bf32c70a224ed6,
title = "CARD15 gene mutations in periodontitis",
abstract = "Objectives: The CARD15 gene encodes the Nod2 protein, which is involved in intracellular recognition of bacterial products like peptidoglycan, activates inflammation and regulates apoptosis through nuclear factor-kappa B, a transcription factor that plays a central role in the innate immunity. Two functional mutations, an insertion mutation at nucleotide 3020 (3020insC) and a missense mutation C2104T in the CARD15 gene (originally NOD2 gene) have been reported to be associated with Crohn's disease. Our aim was to investigate the occurrence of CARD15 gene polymorphisms in adult patients with periodontitis taking into account smoking and presence of putative periodontal pathogens as additional variables.Material and methods: A case-control study was performed in 104 Dutch Caucasian patients with severe adult periodontitis (54 non-smokers and 50 smokers, mean age 46 years) and in 97 ethnically matched, periodontal healthy controls (73 non-smokers and 24 smokers, mean age 40 years). DNA isolated from a mouthwash was typed with PCR technology. Presence of putative periodontal pathogens was established by culture technique.Results: Frequencies of the CARD15 3020insC and 2104T mutations were similar in the periodontitis group and in the control group (5.1% and 13.3%; 5.2% and 10.3%, respectively). The highest carrier frequency of CARD15 mutations was found in non-smoking patients without Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans (29.4% versus 17.4% in controls); however it did not reach statistical significance.Conclusion: Our results suggest no role for CARD15 3020insC and C2104T mutations in adult periodontitis.",
keywords = "Actinobacillus actinomycetemcomitans, adult periodontitis, CARD15, mutation, Porphyromonas gingivalis, smoking, KAPPA-B ACTIVATION, CROHNS-DISEASE, ADULT PERIODONTITIS, ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, NOD2, ASSOCIATION, SUSCEPTIBILITY, POLYMORPHISMS, SMOKING, FAMILY",
author = "ML Laine and LS Murillo and SA Morre and EG Winkel and AS Pena and {van Winkelhoff}, AJ",
year = "2004",
month = oct,
doi = "10.1111/j.1600-051X.2004.00577.x",
language = "English",
volume = "31",
pages = "890--893",
journal = "Journal of Clinical Periodontology",
issn = "0303-6979",
publisher = "Wiley",
number = "10",

}

RIS

TY - JOUR

T1 - CARD15 gene mutations in periodontitis

AU - Laine, ML

AU - Murillo, LS

AU - Morre, SA

AU - Winkel, EG

AU - Pena, AS

AU - van Winkelhoff, AJ

PY - 2004/10

Y1 - 2004/10

N2 - Objectives: The CARD15 gene encodes the Nod2 protein, which is involved in intracellular recognition of bacterial products like peptidoglycan, activates inflammation and regulates apoptosis through nuclear factor-kappa B, a transcription factor that plays a central role in the innate immunity. Two functional mutations, an insertion mutation at nucleotide 3020 (3020insC) and a missense mutation C2104T in the CARD15 gene (originally NOD2 gene) have been reported to be associated with Crohn's disease. Our aim was to investigate the occurrence of CARD15 gene polymorphisms in adult patients with periodontitis taking into account smoking and presence of putative periodontal pathogens as additional variables.Material and methods: A case-control study was performed in 104 Dutch Caucasian patients with severe adult periodontitis (54 non-smokers and 50 smokers, mean age 46 years) and in 97 ethnically matched, periodontal healthy controls (73 non-smokers and 24 smokers, mean age 40 years). DNA isolated from a mouthwash was typed with PCR technology. Presence of putative periodontal pathogens was established by culture technique.Results: Frequencies of the CARD15 3020insC and 2104T mutations were similar in the periodontitis group and in the control group (5.1% and 13.3%; 5.2% and 10.3%, respectively). The highest carrier frequency of CARD15 mutations was found in non-smoking patients without Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans (29.4% versus 17.4% in controls); however it did not reach statistical significance.Conclusion: Our results suggest no role for CARD15 3020insC and C2104T mutations in adult periodontitis.

AB - Objectives: The CARD15 gene encodes the Nod2 protein, which is involved in intracellular recognition of bacterial products like peptidoglycan, activates inflammation and regulates apoptosis through nuclear factor-kappa B, a transcription factor that plays a central role in the innate immunity. Two functional mutations, an insertion mutation at nucleotide 3020 (3020insC) and a missense mutation C2104T in the CARD15 gene (originally NOD2 gene) have been reported to be associated with Crohn's disease. Our aim was to investigate the occurrence of CARD15 gene polymorphisms in adult patients with periodontitis taking into account smoking and presence of putative periodontal pathogens as additional variables.Material and methods: A case-control study was performed in 104 Dutch Caucasian patients with severe adult periodontitis (54 non-smokers and 50 smokers, mean age 46 years) and in 97 ethnically matched, periodontal healthy controls (73 non-smokers and 24 smokers, mean age 40 years). DNA isolated from a mouthwash was typed with PCR technology. Presence of putative periodontal pathogens was established by culture technique.Results: Frequencies of the CARD15 3020insC and 2104T mutations were similar in the periodontitis group and in the control group (5.1% and 13.3%; 5.2% and 10.3%, respectively). The highest carrier frequency of CARD15 mutations was found in non-smoking patients without Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans (29.4% versus 17.4% in controls); however it did not reach statistical significance.Conclusion: Our results suggest no role for CARD15 3020insC and C2104T mutations in adult periodontitis.

KW - Actinobacillus actinomycetemcomitans

KW - adult periodontitis

KW - CARD15

KW - mutation

KW - Porphyromonas gingivalis

KW - smoking

KW - KAPPA-B ACTIVATION

KW - CROHNS-DISEASE

KW - ADULT PERIODONTITIS

KW - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

KW - NOD2

KW - ASSOCIATION

KW - SUSCEPTIBILITY

KW - POLYMORPHISMS

KW - SMOKING

KW - FAMILY

U2 - 10.1111/j.1600-051X.2004.00577.x

DO - 10.1111/j.1600-051X.2004.00577.x

M3 - Article

VL - 31

SP - 890

EP - 893

JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

IS - 10

ER -

ID: 13927333