Can continuous intraperitoneal infusion of I-125-iothalamate and I-131-Hippuran be used for measurement of GFR in conscious rats?de Vries, P. A. M., Navis, G. J., De Jong, P. E. & de Zeeuw, D., 1998, In : Renal Failure. 20, 2, p. 249 - 255 7 p.
Research output: Contribution to journal › Article › Academic › peer-review
We previously described a method to measure GFR in conscious spontaneously voiding rats. This method circumvents the need for anesthesia and for bladder instrumentation. If's main principle is the correction of renal I-125-iothalamate clearance for incomplete urine collection by the ratio of plasma and venal clearance of co-infused I-131-Hippuran. A disadvantage of this technique is the requirement of an infra-arterial catheter for infusion of the renal function tracers. We therefore tested whether intraperitoneal infusion of I-125-iothalamate and I-131-Hippuran can be used far such a GFR measurement in conscious spontaneously voiding rats. We found that during intraperitoneal administration, stable plasma levels of I-131-Hippuran could be obtained. However, urinary recovery of I-131-Hippuran was incomplete (66 +/- 32%), leading to a significant overestimation of GFR by 140 +/- 13% in comparison with the GFR measured by the intra-arterial technique. Thus intraperitoneal infusion of renal function tracers cannot replace intra-arterial infusion.
|Pages (from-to)||249 - 255|
|Number of pages||7|
|Publication status||Published - 1998|
|Event||8th International Symposium of Nephrology - , Italy|
Duration: 7-Oct-1996 → 9-Oct-1996
8th International Symposium of Nephrology
07/10/1996 → 09/10/1996Italy
- I-125-iothalamate, I-131-Hippuran, glomerular filtration rate, GLOMERULAR-FILTRATION RATE, RENAL HEMODYNAMICS, ANESTHESIA, FLOW