Publication

Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta

Gouw, M. A. M., Wilffert, B., Wermelskirchen, D. & Van Zwieten, P. A., 22-Oct-1990, In : Pharmacology. 40, 5, p. 277-287 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Gouw, M. A. M., Wilffert, B., Wermelskirchen, D., & Van Zwieten, P. A. (1990). Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta. Pharmacology, 40(5), 277-287.

Author

Gouw, M.A.M. ; Wilffert, B. ; Wermelskirchen, D. ; Van Zwieten, P.A. / Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta. In: Pharmacology. 1990 ; Vol. 40, No. 5. pp. 277-287.

Harvard

Gouw, MAM, Wilffert, B, Wermelskirchen, D & Van Zwieten, PA 1990, 'Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta' Pharmacology, vol. 40, no. 5, pp. 277-287.

Standard

Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta. / Gouw, M.A.M.; Wilffert, B.; Wermelskirchen, D.; Van Zwieten, P.A.

In: Pharmacology, Vol. 40, No. 5, 22.10.1990, p. 277-287.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Gouw MAM, Wilffert B, Wermelskirchen D, Van Zwieten PA. Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta. Pharmacology. 1990 Oct 22;40(5):277-287.


BibTeX

@article{0233849aaaac4b7dbf4772502fcca87a,
title = "Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta",
abstract = "We determined the contribution of intracellular Ca2+to the noradrenaline (NA, 3 x 10-5mmol/l)-induced contraction of the isolated guinea pig aorta. Since only about 55{\%} of the NA-induced contraction could be attributed to intracellular Ca2+release, we assumed that a Ca2+influx component contributes to the NA-induced contraction. This influx component proved resistant to the organic calcium entry blockers (CEBs) nifedipine, diltiazem, flunarizine and gallopamil which, in contrast, antagonized K+-induced Ca2+influx completely. Conversely, the NA-induced Ca2+influx component could be antagonized by the inorganic cations La3+, Cd2+, Mn2+, Ni2+and Co2+.45Ca2+uptake experiments also revealed that both KCl and NA induce Ca2+influx of which only the latter one is resistant to nifedipine. It was concluded that in the guinea pig aorta NA activates a receptor-operated channel through which Ca2+can be translocated from the extracellular space to the cytosol to contribute directly to contraction.",
keywords = "45Ca2+uptake, agonist-sensitive calcium pool, Ca2+entry blockade, Ca2+influx, guinea pig aorta, noradrenaline, aminophenol, cadmium, calcium 45, calcium antagonist, cobalt, diltiazem, egtazic acid, flunarizine, gallopamil, lanthanum, manganese, nickel, nifedipine, noradrenalin, radioisotope, animal cell, aorta, article, calcium transport, guinea pig, male, nonhuman, priority journal, smooth muscle contraction",
author = "M.A.M. Gouw and B. Wilffert and D. Wermelskirchen and {Van Zwieten}, P.A.",
year = "1990",
month = "10",
day = "22",
language = "English",
volume = "40",
pages = "277--287",
journal = "Pharmacology",
issn = "0031-7012",
number = "5",

}

RIS

TY - JOUR

T1 - Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta

AU - Gouw, M.A.M.

AU - Wilffert, B.

AU - Wermelskirchen, D.

AU - Van Zwieten, P.A.

PY - 1990/10/22

Y1 - 1990/10/22

N2 - We determined the contribution of intracellular Ca2+to the noradrenaline (NA, 3 x 10-5mmol/l)-induced contraction of the isolated guinea pig aorta. Since only about 55% of the NA-induced contraction could be attributed to intracellular Ca2+release, we assumed that a Ca2+influx component contributes to the NA-induced contraction. This influx component proved resistant to the organic calcium entry blockers (CEBs) nifedipine, diltiazem, flunarizine and gallopamil which, in contrast, antagonized K+-induced Ca2+influx completely. Conversely, the NA-induced Ca2+influx component could be antagonized by the inorganic cations La3+, Cd2+, Mn2+, Ni2+and Co2+.45Ca2+uptake experiments also revealed that both KCl and NA induce Ca2+influx of which only the latter one is resistant to nifedipine. It was concluded that in the guinea pig aorta NA activates a receptor-operated channel through which Ca2+can be translocated from the extracellular space to the cytosol to contribute directly to contraction.

AB - We determined the contribution of intracellular Ca2+to the noradrenaline (NA, 3 x 10-5mmol/l)-induced contraction of the isolated guinea pig aorta. Since only about 55% of the NA-induced contraction could be attributed to intracellular Ca2+release, we assumed that a Ca2+influx component contributes to the NA-induced contraction. This influx component proved resistant to the organic calcium entry blockers (CEBs) nifedipine, diltiazem, flunarizine and gallopamil which, in contrast, antagonized K+-induced Ca2+influx completely. Conversely, the NA-induced Ca2+influx component could be antagonized by the inorganic cations La3+, Cd2+, Mn2+, Ni2+and Co2+.45Ca2+uptake experiments also revealed that both KCl and NA induce Ca2+influx of which only the latter one is resistant to nifedipine. It was concluded that in the guinea pig aorta NA activates a receptor-operated channel through which Ca2+can be translocated from the extracellular space to the cytosol to contribute directly to contraction.

KW - 45Ca2+uptake

KW - agonist-sensitive calcium pool

KW - Ca2+entry blockade

KW - Ca2+influx

KW - guinea pig aorta

KW - noradrenaline

KW - aminophenol

KW - cadmium

KW - calcium 45

KW - calcium antagonist

KW - cobalt

KW - diltiazem

KW - egtazic acid

KW - flunarizine

KW - gallopamil

KW - lanthanum

KW - manganese

KW - nickel

KW - nifedipine

KW - noradrenalin

KW - radioisotope

KW - animal cell

KW - aorta

KW - article

KW - calcium transport

KW - guinea pig

KW - male

KW - nonhuman

KW - priority journal

KW - smooth muscle contraction

M3 - Article

VL - 40

SP - 277

EP - 287

JO - Pharmacology

JF - Pharmacology

SN - 0031-7012

IS - 5

ER -

ID: 14137304