Publication

C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques

Koopman, M., Seinstra, R. I. & Nollen, E. A. A., 2019, Alpha-Synuclein. Bartels, T. (ed.). p. 93-112 20 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

APA

Koopman, M., Seinstra, R. I., & Nollen, E. A. A. (2019). C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques. In T. Bartels (Ed.), Alpha-Synuclein (pp. 93-112). (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9124-2_9

Author

Koopman, Mandy ; Seinstra, Renée I ; Nollen, Ellen A A. / C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases : Tools and Techniques. Alpha-Synuclein. editor / Tim Bartels. 2019. pp. 93-112 (Methods in Molecular Biology).

Harvard

Koopman, M, Seinstra, RI & Nollen, EAA 2019, C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques. in T Bartels (ed.), Alpha-Synuclein. Methods in Molecular Biology, pp. 93-112. https://doi.org/10.1007/978-1-4939-9124-2_9

Standard

C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases : Tools and Techniques. / Koopman, Mandy; Seinstra, Renée I; Nollen, Ellen A A.

Alpha-Synuclein. ed. / Tim Bartels. 2019. p. 93-112 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Vancouver

Koopman M, Seinstra RI, Nollen EAA. C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques. In Bartels T, editor, Alpha-Synuclein. 2019. p. 93-112. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9124-2_9


BibTeX

@inbook{eac412ca34a743d49772700228061d04,
title = "C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques",
abstract = "Caenorhabditis elegans is widely used to investigate biological processes related to health and disease. Multiple C. elegans models for human neurodegenerative diseases do exist, including those expressing human α-synuclein. Even though these models do not feature all pathological and molecular hallmarks of the disease they mimic, they allow for the identification and dissection of molecular pathways that are involved. In line with this, genetic screens have yielded multiple modifiers of proteotoxicity in C. elegans models for neurodegenerative diseases. Here, we describe a set of common screening approaches and tools that can be used to study synucleinopathies and other neurodegenerative diseases in C. elegans. RNA interference and mutagenesis screens can be used to find genes that affect proteotoxicity, while relatively simple molecular, cellular (fractionation studies), metabolic (respiration studies), and behavioral (thrashing and crawling) readouts can be used to study the effects of disease proteins and modifiers more closely.",
author = "Mandy Koopman and Seinstra, {Ren{\'e}e I} and Nollen, {Ellen A A}",
year = "2019",
doi = "10.1007/978-1-4939-9124-2_9",
language = "English",
isbn = "978-1-4939-9123-5",
series = "Methods in Molecular Biology",
pages = "93--112",
editor = "Tim Bartels",
booktitle = "Alpha-Synuclein",

}

RIS

TY - CHAP

T1 - C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases

T2 - Tools and Techniques

AU - Koopman, Mandy

AU - Seinstra, Renée I

AU - Nollen, Ellen A A

PY - 2019

Y1 - 2019

N2 - Caenorhabditis elegans is widely used to investigate biological processes related to health and disease. Multiple C. elegans models for human neurodegenerative diseases do exist, including those expressing human α-synuclein. Even though these models do not feature all pathological and molecular hallmarks of the disease they mimic, they allow for the identification and dissection of molecular pathways that are involved. In line with this, genetic screens have yielded multiple modifiers of proteotoxicity in C. elegans models for neurodegenerative diseases. Here, we describe a set of common screening approaches and tools that can be used to study synucleinopathies and other neurodegenerative diseases in C. elegans. RNA interference and mutagenesis screens can be used to find genes that affect proteotoxicity, while relatively simple molecular, cellular (fractionation studies), metabolic (respiration studies), and behavioral (thrashing and crawling) readouts can be used to study the effects of disease proteins and modifiers more closely.

AB - Caenorhabditis elegans is widely used to investigate biological processes related to health and disease. Multiple C. elegans models for human neurodegenerative diseases do exist, including those expressing human α-synuclein. Even though these models do not feature all pathological and molecular hallmarks of the disease they mimic, they allow for the identification and dissection of molecular pathways that are involved. In line with this, genetic screens have yielded multiple modifiers of proteotoxicity in C. elegans models for neurodegenerative diseases. Here, we describe a set of common screening approaches and tools that can be used to study synucleinopathies and other neurodegenerative diseases in C. elegans. RNA interference and mutagenesis screens can be used to find genes that affect proteotoxicity, while relatively simple molecular, cellular (fractionation studies), metabolic (respiration studies), and behavioral (thrashing and crawling) readouts can be used to study the effects of disease proteins and modifiers more closely.

U2 - 10.1007/978-1-4939-9124-2_9

DO - 10.1007/978-1-4939-9124-2_9

M3 - Chapter

SN - 978-1-4939-9123-5

T3 - Methods in Molecular Biology

SP - 93

EP - 112

BT - Alpha-Synuclein

A2 - Bartels, Tim

ER -

ID: 84195995