Publication

Bridging fluorescence microscopy and electron microscopy

Giepmans, B. N. G., Aug-2008, In : Histochemistry and cell biology. 130, 2, p. 211-217 7 p.

Research output: Contribution to journalReview articleAcademicpeer-review

Development of new fluorescent probes and fluorescence microscopes has led to new ways to study cell biology. With the emergence of specialized microscopy units at most universities and research centers, the use of these techniques is well within reach for a broad research community. A major breakthrough in fluorescence microscopy in biology is the ability to follow specific targets on or in living cells, revealing dynamic localization and/or function of target molecules. One of the inherent limitations of fluorescence microscopy is the resolution. Several efforts are undertaken to overcome this limit. The traditional and most well-known way to achieve higher resolution imaging is by electron microscopy. Moreover, electron microscopy reveals organelles, membranes, macromolecules, and thus aids in the understanding of cellular complexity and localization of molecules of interest in relation to other structures. With the new probe development, a solid bridge between fluorescence microscopy and electron microscopy is being built, even leading to correlative imaging. This connection provides several benefits, both scientifically as well as practically. Here, I summarize recent developments in bridging microscopy.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalHistochemistry and cell biology
Volume130
Issue number2
Publication statusPublished - Aug-2008

    Keywords

  • fluorescent proteins, tetracysteine biarsenical system, Quantum dots, correlative light and electron microscopy, Golgi apparatus, LIVING CELLS, QUANTUM DOTS, CORRELATED LIGHT, LIVE CELLS, PROTEIN, RED, PHOTOOXIDATION, TRAFFICKING, MOLECULES, MARKERS

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