breakpointR: an R/Bioconductor package to localize strand state changes in Strand-seq dataPorubsky, D., Sanders, A. D., Taudt, A., Colomé-Tatché, M., Lansdorp, P. M. & Guryev, V., 30-Aug-2019, In : Bioinformatics (Oxford, England).
Research output: Contribution to journal › Article › Academic › peer-review
MOTIVATION: Strand-seq is a specialized single-cell DNA sequencing technique centered around the directionality of single-stranded DNA. Computational tools for Strand-seq analyses must capture the strand-specific information embedded in these data.
RESULTS: Here we introduce breakpointR, an R/Bioconductor package specifically tailored to process and interpret single-cell strand-specific sequencing data obtained from Strand-seq. We developed breakpointR to detect local changes in strand directionality of aligned Strand-seq data, to enable fine-mapping of sister chromatid exchanges, germline inversion and to support global haplotype assembly. Given the broad spectrum of Strand-seq applications we expect breakpointR to be an important addition to currently available tools and extend the accessibility of this novel sequencing technique.
AVAILABILITY: R/Bioconductor package https://bioconductor.org/packages/breakpointR.
|Journal||Bioinformatics (Oxford, England)|
|Publication status||E-pub ahead of print - 30-Aug-2019|