Publication

Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster

Cogut, V., Bruintjes, J. J., Eggen, B. J. L., van der Zee, E. A. & Henning, R. H., Feb-2018, In : Brain behavior and immunity. 68, p. 17-22 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Cogut, V., Bruintjes, J. J., Eggen, B. J. L., van der Zee, E. A., & Henning, R. H. (2018). Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster. Brain behavior and immunity, 68, 17-22. https://doi.org/10.1016/j.bbi.2017.10.009

Author

Cogut, V. ; Bruintjes, J. J. ; Eggen, B. J. L. ; van der Zee, E. A. ; Henning, R. H. / Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster. In: Brain behavior and immunity. 2018 ; Vol. 68. pp. 17-22.

Harvard

Cogut, V, Bruintjes, JJ, Eggen, BJL, van der Zee, EA & Henning, RH 2018, 'Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster', Brain behavior and immunity, vol. 68, pp. 17-22. https://doi.org/10.1016/j.bbi.2017.10.009

Standard

Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster. / Cogut, V.; Bruintjes, J. J.; Eggen, B. J. L.; van der Zee, E. A.; Henning, R. H.

In: Brain behavior and immunity, Vol. 68, 02.2018, p. 17-22.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Cogut V, Bruintjes JJ, Eggen BJL, van der Zee EA, Henning RH. Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster. Brain behavior and immunity. 2018 Feb;68:17-22. https://doi.org/10.1016/j.bbi.2017.10.009


BibTeX

@article{aa5821365d444c6d88ea2c5d880a03c9,
title = "Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster",
abstract = "Hibernators tolerate low metabolism, reduced cerebral blood flow and hypothermia during torpor without noticeable neuronal or synaptic dysfunction upon arousal. Previous studies found extensive changes in brain during torpor, including synaptic rearrangements, documented both morphologically and molecularly. As such adaptations may represent organ damage, we anticipated an inflammatory response in brain during specific hibernation phases. In this study, signs of inflammation in the brain were investigated in the Syrian hamster hippocampus (Mesocricetus Auratus) both during hibernation (torpor and arousal phases) and in summer and winter euthermic animals.mRNA expression of the pro-inflammatory cytokines TNF-alpha, IL-6 and IL-1 beta was quantified by RT-qPCR. Morphological changes of microglia were studied by immunohistochemistry staining for IBA-I. Activation of microglia based on retraction and thickening of the dendritic branches and an increase in cell body size was quantified by calculation of cell body size to total cell size ratio.Expression of pro-inflammatory cytokines was upregulated early in arousal (90 min), and normalized after 8 h of arousal. Substantial loss of microglia ramification was found throughout torpor and early arousal together with a 2-fold increase in the cell body size to total cell size ratio. Notably, microglia changes were fully reversed in late arousal (8 h) to euthermic levels.These results demonstrate an upregulation of inflammatory cytokines and signs of microglia activation during hibernation, which completely resolves by late arousal. Activation of this response may serve to prevent or offset brain damage resulting from the substantial physiological changes accompanying torpor and their rapid change during early arousal. (C) 2017 Elsevier Inc. All rights reserved.",
keywords = "Brain, Microglia, Inflammatory cytokines, Neuroinflammation, Hibernation, Syrian hamster, ARCTIC GROUND-SQUIRREL, NEURODEGENERATIVE DISEASES, MAMMALIAN HIBERNATION, CEREBRAL-ISCHEMIA, CARDIAC-ARREST, HYPOTHERMIA, INJURY, TOLERANCE, NEUROPROTECTION, TEMPERATURE",
author = "V. Cogut and Bruintjes, {J. J.} and Eggen, {B. J. L.} and {van der Zee}, {E. A.} and Henning, {R. H.}",
note = "Copyright {\circledC} 2017 Elsevier Inc. All rights reserved.",
year = "2018",
month = "2",
doi = "10.1016/j.bbi.2017.10.009",
language = "English",
volume = "68",
pages = "17--22",
journal = "Brain, Behavior, and Immunity",
issn = "0889-1591",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",

}

RIS

TY - JOUR

T1 - Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster

AU - Cogut, V.

AU - Bruintjes, J. J.

AU - Eggen, B. J. L.

AU - van der Zee, E. A.

AU - Henning, R. H.

N1 - Copyright © 2017 Elsevier Inc. All rights reserved.

PY - 2018/2

Y1 - 2018/2

N2 - Hibernators tolerate low metabolism, reduced cerebral blood flow and hypothermia during torpor without noticeable neuronal or synaptic dysfunction upon arousal. Previous studies found extensive changes in brain during torpor, including synaptic rearrangements, documented both morphologically and molecularly. As such adaptations may represent organ damage, we anticipated an inflammatory response in brain during specific hibernation phases. In this study, signs of inflammation in the brain were investigated in the Syrian hamster hippocampus (Mesocricetus Auratus) both during hibernation (torpor and arousal phases) and in summer and winter euthermic animals.mRNA expression of the pro-inflammatory cytokines TNF-alpha, IL-6 and IL-1 beta was quantified by RT-qPCR. Morphological changes of microglia were studied by immunohistochemistry staining for IBA-I. Activation of microglia based on retraction and thickening of the dendritic branches and an increase in cell body size was quantified by calculation of cell body size to total cell size ratio.Expression of pro-inflammatory cytokines was upregulated early in arousal (90 min), and normalized after 8 h of arousal. Substantial loss of microglia ramification was found throughout torpor and early arousal together with a 2-fold increase in the cell body size to total cell size ratio. Notably, microglia changes were fully reversed in late arousal (8 h) to euthermic levels.These results demonstrate an upregulation of inflammatory cytokines and signs of microglia activation during hibernation, which completely resolves by late arousal. Activation of this response may serve to prevent or offset brain damage resulting from the substantial physiological changes accompanying torpor and their rapid change during early arousal. (C) 2017 Elsevier Inc. All rights reserved.

AB - Hibernators tolerate low metabolism, reduced cerebral blood flow and hypothermia during torpor without noticeable neuronal or synaptic dysfunction upon arousal. Previous studies found extensive changes in brain during torpor, including synaptic rearrangements, documented both morphologically and molecularly. As such adaptations may represent organ damage, we anticipated an inflammatory response in brain during specific hibernation phases. In this study, signs of inflammation in the brain were investigated in the Syrian hamster hippocampus (Mesocricetus Auratus) both during hibernation (torpor and arousal phases) and in summer and winter euthermic animals.mRNA expression of the pro-inflammatory cytokines TNF-alpha, IL-6 and IL-1 beta was quantified by RT-qPCR. Morphological changes of microglia were studied by immunohistochemistry staining for IBA-I. Activation of microglia based on retraction and thickening of the dendritic branches and an increase in cell body size was quantified by calculation of cell body size to total cell size ratio.Expression of pro-inflammatory cytokines was upregulated early in arousal (90 min), and normalized after 8 h of arousal. Substantial loss of microglia ramification was found throughout torpor and early arousal together with a 2-fold increase in the cell body size to total cell size ratio. Notably, microglia changes were fully reversed in late arousal (8 h) to euthermic levels.These results demonstrate an upregulation of inflammatory cytokines and signs of microglia activation during hibernation, which completely resolves by late arousal. Activation of this response may serve to prevent or offset brain damage resulting from the substantial physiological changes accompanying torpor and their rapid change during early arousal. (C) 2017 Elsevier Inc. All rights reserved.

KW - Brain

KW - Microglia

KW - Inflammatory cytokines

KW - Neuroinflammation

KW - Hibernation

KW - Syrian hamster

KW - ARCTIC GROUND-SQUIRREL

KW - NEURODEGENERATIVE DISEASES

KW - MAMMALIAN HIBERNATION

KW - CEREBRAL-ISCHEMIA

KW - CARDIAC-ARREST

KW - HYPOTHERMIA

KW - INJURY

KW - TOLERANCE

KW - NEUROPROTECTION

KW - TEMPERATURE

U2 - 10.1016/j.bbi.2017.10.009

DO - 10.1016/j.bbi.2017.10.009

M3 - Article

C2 - 29038037

VL - 68

SP - 17

EP - 22

JO - Brain, Behavior, and Immunity

JF - Brain, Behavior, and Immunity

SN - 0889-1591

ER -

ID: 54764115