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Brain death induces renal expression of heme oxygenase-1 and heat shock protein 70

van Dullemen, L. F. A., Bos, E. M., Schuurs, T. A., Kampinga, H. H., Ploeg, R. J., van Goor, H. & Leuvenink, H. G. D., 29-Jan-2013, In : Journal of translational medicine. 11, 10 p., 22.

Research output: Contribution to journalArticleAcademicpeer-review

Background: Kidneys derived from brain dead donors have lower graft survival and higher graft-function loss compared to their living donor counterpart. Heat Shock Proteins (HSP) are a large family of stress proteins involved in maintaining cell homeostasis. We studied the role of stress-inducible genes Heme Oxygenase-1 (HO-1), HSP27, HSP40, and HSP70 in the kidney following a 4 hour period of brain death.

Methods: Brain death was induced in rats (n=6) by inflating a balloon catheter in the epidural space. Kidneys were analysed for HSPs using RT-PCR, Western blotting, and immunohistochemistry.

Results: RT-PCR data showed a significant increase in gene expression for HO-1 and HSP70 in kidneys of brain dead rats. Western blotting revealed a massive increase in HO-1 protein in brain dead rat kidneys. Immunohistochemistry confirmed these findings, showing extensive HO-1 protein expression in the renal cortical tubules of brain dead rats. HSP70 protein was predominantly increased in renal distal tubules of brain dead rats treated for hypotension.

Conclusion: Renal stress caused by brain death induces expression of the cytoprotective genes HO-1 and HSP70, but not of HSP27 and HSP40. The upregulation of these cytoprotective genes indicate that renal damage occurs during brain death, and could be part of a protective or recuperative mechanism induced by brain death-associated stress.

Original languageEnglish
Article number22
Number of pages10
JournalJournal of translational medicine
Volume11
Publication statusPublished - 29-Jan-2013

    Keywords

  • Kidney, Protective genes, Rat, Organ donation, HSP, HSP70, HSP40, HSP27, ISCHEMIA-REPERFUSION INJURY, HEAT-SHOCK PROTEINS, KIDNEY GRAFT FUNCTION, DONOR KIDNEYS, ISCHEMIA/REPERFUSION INJURY, IN-VIVO, INDUCTION, RAT, SURVIVAL, TRANSPLANTATION

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